Effects Of Naringin To The Proliferation And SOD,NOS Of Rabbit Articular Chondrocytes Which Are Cultured In Vitro

Objective: To observe the effects of Naringin of Rhizoma Drynaria to theproliferation of rabbit articular chondrocytes which are normal and damaged byTNF-a and monolayer cultured in vitro. To observe the influence of Naringin to SODand NOS ofchondrocytes which are damaged by TNF-α. To probe the prevention andcure mechanisms of Rhizoma Drynaria to osteoarthritis (OA) in the direction ofarticular chondrocytes, so as to provide experimental evidence for Rhizoma Drynariacuring OA.Methods:1. The rabbit articular chondrocytes were demeshed and cultured by DMEM(include 10%FBS). The attached cells was observed by inverted microscope, thendraw the growth curve through MTT assay, and identified according to toluidine bluestain.2. The proliferation rates of chondrocytes which were normal and damaged byTNF-αwere determined by MTT assay.3. The chondrocytes were induced by TNF-αand the content of SOD and NOSin supernate are tested.Results:1. The origin generation of chondrocytes were mostly adhering to the wall aftercultured 48 hours, and can pass to the next generation about 7 days. The cells could bedyed into amethyst in the toluidine blue stain, and the extracellular matrix could bedyed into light blue.2. Several concentrations of Naringin could obviously promote the proliferationof normal chondrocytes, and had significant difference compared to the blank controlgroup(P＜0.05). They were concentrations of 100μg/ml, 50μg/ml, 25μg/ml and12.5μg/ml. The concentrations of 12.5μg/ml Naringin had the best promotion amongthem; Several concentrations of Naringin could obviously promote the proliferation of chondrocytes induced by TNF-α, and had remarkable difference compared to theexperimental control group (P＜0.05). They were concentrations of 200μg/ml,100μg/ml, 50μg/ml, and 25μg/ml. The concentrations of 25μg/ml Naringin had thebest promotion among them.3. The chondrocytes' SOD activity of every Naringin group was higher than boththe blank control group and the experimental control group, and had significantdifference compared to the blank control group and the experimental controlgroup(P＜0.05). The chondrocytes' NOS activity of every Naringin group was higherthan the blank control group, lower than the experimental control group. Compared tothe experimental control group, the NOS activity of every Naringin group hadsignificant difference(P＜0.05).Conclusion:1. The toluidine blue stain can identify chondrocytes.2. Certain concentration of Naringin can promote the proliferation ofchondrocytes.3. Naringin can enhance the chondrocytes' SOD activity and lower thechondrocytes' NOS activity remarkably, and can degrade the impairment of TNF-αtochondrocytes.