α-adducin And Angiotensin Converting Enzyme Polymorphisms In Salt-sensitive Hypertension And Renal Injury

Objective To investigate the association between theα-adducin Gly460Trp,ACE I/D polymorphisms and salt-sensitive hypertension,ren al injury in Chinese people; To investigate the effects of oral acute saline load on renal production of prostaglandin I2(PGI2) and thromboxane A2(TXA2) in salt-sensitive hypertensive patient.Methods The case-control study was performed in 150 hypertensive and 150 normal tensive(NT) subjects in china, A previous medical history of hypertension and body mass index (BMI) was recorded. Fasting plasma glucose, triglyceride, and cholesterol were measured; 150 patients with essential hypertension were divided into salt-sensitive( SS =83) and non-salt-sensitive(NSS=34) groups by modified Sullivan's method, The Genotypes ofα-adducin gene were determined by Restriction Fragment Length Polymorphism-Polymerase Chain Reaction(RFLP-PCR) and AC E genotypes were determined by polymerase chain reaction(PCR)-agarose gelelectrophoresis; To measurePRA, blood and urineβ₂-Mg and urine ALB in 150 essential hypertension subjects, Using radio-immunity to test levels of 6-keto-PGF_1α,TXB₂,TXB₂/PGF_1α before and after acute saline load in 150 hypertensive patients.Results (1) A higher prevalence of a- adducin G460T TT and T allele among patients with essentialhy pretension was noted(P＜0.05). No significant differences were found between patients with essential hypertension and normotensive control subjects in neither genotype nor allele frequencies of the ACE gene I/D polymorphism (P＞0.05); In SS and NSS groups,There were statistical significance of polymorphism ofα-adducin G460T TT genotype, ACE I allele gene and TT+I combined genotype between SS and NSS subjects(P＜0.05), but there were no statistical significance between the level of PRA and a- adducin , ACE genotype (P＞0.05). (2) The levels of urineβ₂-MG and ALB/Cr in SS patients were higher than NSS patients,there was statistical significance(P＜0.05), but there were no statistical significance of the level of bloodβ₂-Mg (P＞0.05). (3)In SS group, The levels of urineβ₂-MG and ALB/Cr in group of ACEâ…¡genotype were higher than ACE ID and DD genotype, The levels of urineβ₂-MG and ALB/Cr in group of a-adducin G460T TT genotype were higher thanα-adducin G460T GT and GG genotype, there was statistical significance(P＜0.05). (4) after oral acute saline load,the levels of 6-keto-PGF_1α were lower in salt-ensitive(SS) hypertensive patients than that in-nonsalt-sensitive(NSS) ones, and the decrease from baseline was greater in SS group than that in NSS group ,Both the levels of TXB₂,TXB₂/PGF_1α and the increase in the levels of TXB₂,TXB₂/PGF_1α were greater in SS hypertensive patients than those in NSS ones after salt loading, but there were statistical significance in the levels of PGF_1α and TXB₂/PGF_1α after two h our of salt loading.Conclusion (1)Theα-adducin G460T TT genotype and ACE I allele gene are significantly associated with SS hypertension. (2)α-adducin G460T TT genotype, ACEâ…¡genotype are significantly associated with SS hypertension accomping renal ingury. There are may be a risk for hypertensive kidney lesion. (3)Saline load produces significantly different effects on renal production of PGI₂ and TXA₂ in SS and NSS hypertensive patients, and these changes may be related to the pathophysiology of SS hypertensive patients after acute salt loading.