| objective to observe the effect of hepatocyte growth factor for preserving isolated heart of rats .Methods fourty rats were random divided into two groups :.Control group (n=20).isolated rat hearts were preserved in 4℃HTK solution . Experimental group (n=20).isolated rat hearts were preserved in 4℃HTK+rh-HGF(100μg/L)solution. Before and subjected to 8 hr (group A,C), 12 hr (group B,D) of hypothermic storage followed by 5 min of normothermic reperfusion on modified Langendorff-Neely model . The recovery rate of cardiac function afer myocardial preservation,such as LVSP and LVDP and±dp/dtmax and the changes of CSF ,CK,LDH,NO and ET were measured . Cell apoptosis was deteced by TUNEL staining and the expression of c-met during myocardial preservation was analyzed by immunohistochemical. The expression change of bcl-2mRNA was examined by RT-PCR.Results Compared with Control group, Experimental group showed a significantly higher recovery rate of LVSP,LVDP and±dp/dtmax ( P<0.01) and the leakage of CK,LDH was significant lower than that of control group( P<0.01) anda lower rate of TUNEL-positive cardiomyocytes ( P<0.01) after reperfusion. Immunohistochemical analysis revealed that c-met receptor expression was stronger in the HGF-treated myocardium than in the non-HGF-treated myocardium after storage and was associated with a stronger expression of bcl-2 mRNA.Conclusion The administration of rh-HGF before storage improved cardiac function after prolonged myocardial preservation by preventing apoptosis and enhancing expression of bcl-2 mRNA through the c-Met/HGF receptor. Thus, the addition of rh-HGF in the storage solution may be a promising strategy for prolonged heart graft preservation.
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