The Study Of Galla Chinesis Extract On Remineralization Of Root Carious Lesion

Dental caries is a multi-factorial and chronic bacterial disease that involves the destruction of tooth hard tissue structure. It directly results from acid dissolution of the mineral of the teeth. The current concept regarding cariogenesis is that a caries lesion, either clinically invisible or detectable, is the accumulation of numerous de-and re-mineralization , rather than a unidirectional de-mineralization process. If the re-mineralization progresses predominantly over periods, the de-mineralized lesion is to be reversible. Therefore, re-mineralization is an important natural repair process, countering cariogenic challenges. As a chemotherapeutic agent to combat dental caries, fluoride occupies a unique position. Although Fluoride's anti-caries efficacy, i.e., inhibition of tooth demineralization , promotion of incipient lesion re-mineralization , is well-proven, there are a number of reasons to continue to seek alternative, effective non-fluoride anti-caries agents. In our previous study, we find out the water extract of Galla Chinesis has significant anti-caries effects in vitro and in bio-film model. Galla Chinensis had anti-caries prosperities to inhibit the growth and acid production of some kinds of major cariogenic bacteria, and had an ability to inhibit enamel demineralization and enhance the re-mineralization of enamel and root caries in vitro.Root caries, one of the most popular oral diseases in the elders, affects the oral and general health of old people seriously. The combined effects of acids and proteases produced by cariogenic bacteria can lead the demineralization and organic matrix degradation of root tissue. Considering the characterization of the ecologic environment, the histology and pathology of root tissue, the prevention of root caries is more difficult than that of coronary caries. However, the study of Galla Chinesis extract on re-mineralization of different lever root lesions is scarce in our nation. Some has found that dentin phosphoprotein (DPP) has relationship with the re-mineralization of root caries. But how dentin phosphoprotein (DPP) affect the re-mineralization ability of Galla Chinesis extract is remaining obscure till now. The aim of this study is to evaluate the re-mineralization ability of Galla Chinesis extract in different level and whether the re-mineralization ability of Galla Chinesis extract is affected by dentin phosphoprotein (DPP).MethedThe re-mineralization ability of Galla Chinesis extract on the artificial carious lesion in bovine root de-mineralized by two levels of demineralization solutions in vitro were evaluated. These specimens was observed by microscope and scanning electron microscope and compared in histologic pathology. Based on previous study, Galla Chinensis extract were used. Bovine root blocks with different lesions were prepared and randomly divided into treatment groups. Different treatment groups were submitted to pH-cycling. Some other Bovine root specimens after de-mineralized by two levels of demineralization solutions emerged in the solution of 0.5% NaCl. Then the re-mineralization ability of Galla Chinesis extract on the specimens were evaluated. At last, treated specimens of the two experiments were analysed by LSCM.Results1. the re-mineralization ability of Galla Chinesis extract on the artificial carious lesion in bovine root specimen de-mineralized by two levels of demineralization solutions in vitro were apparent and enhance the re-mineralization of the de-mineralized root specimen. The re-mineralization lever of subsurface lesion was better than that of the erosion lesion. The re-mineralization solution could enhance the re-mineralization of the de-mineralized root specimen, less than the re-mineralization ability of Galla Chinesis extract at the erosion lesion.2 .after extracted by 0.5% NaCl, the re-mineralization lever of subsurface lesion were less than that of the erosion lesion. The re-mineralization solution could enhance the re-mineralization of the de-mineralized root specimen, too. But the re-mineralization lever of two re-mineralization solution were not different.ConclusionGalla Chinesis extract can enhance the re-mineralization lever of different root caries. Specimens extracted by 0.5% NaCl can improve the re-mineralization potent especially the erosion lesion .This contribute to the clinic application of root caries treatment. This findings support the proposition that Galla Chinesis may be a promising anti-caries natural medicine in the future.