Galla Chinensis Extract Against Staphylococcus Aureus Remove Titanium Surface Of The Biofilm Efficiency

Objective:To establish a titanium surface of Staphylococcus aureus of the biofilm in vitro model; of Galla chinensis extract in vitro on the clean titanium surface Staphylococcus aureus biofilm synergy.Methods:Select X387 type staphylococcus aureus, in vitro establish titanium plate surface BF model. Use test tube 2-fold dilution the lowest tested drugs bacteriostatic concentration. Will WuBeiZi extract, klingenberg alone or every medicine jinggangmeisu joint action of each other in titanium plate surface with BF, CLSM respectively observed in different drug group intervention 4,8,12,24 h titanium plate surface and staphylococcus aureus biofilm formation conditions. In different locations along with CLSM Z axis scanned, measuring the thickness and structure.Results:According to the experiment, GC The MIC of AS is 32mg/ml, CM MIC of AS, as 2mg/ml. GC, CM inhibit and damage the surface of titanium BF. Observed under the CLSM to BBF from the red, green and yellow fluorescence, of which red fluorescence for the dead bacteria, as viable cells green, yellow, dead cells and viable phase for the superposition. In 4,8,12,24 h, GC groups, CM groups, GC+CM group, NS group under, AS biofilm morphology, significant differences in the structure. Titanium surface bacteria by the experimental group scattered gradually becomes dense, forming a simple corporation or flake-like structure, but formed a typical biofilm structure. NS Group titanium surface bacteria by the loose-intensive and progressive thickening of the biofilm formation of the more typical three-dimensional structure. At different time points, the use of technology-specific fluorescence labeling and CLSM observation of the experimental group AS biofilm thickness of the titanium surface, dead bacteria, the number of viable cells and calculate the percentage of viable cells. Titanium surface of the experimental group showed the thickness of biofilm NS AS group, was statistically significant (P<0.05); the percentage of viable cells compared with the NS group, there was statistically significant (P<0.01).Conclusion:Established subject in the titanium surface of the biofilm formation of AS observed Galla chinensis extract, clindamycin, and the combination of different times after the inhibition of bacterial biofilm and the biofilm on the AS form, structure, activity and implications. Galla chinensis extract on the titanium surface AS biofilm formation processes of early inhibition and can reduce the biofilm activity, biofilm structure of AS changed, undermining its structural integrity. Galla chinensis extract in combination with clindamycin titanium surface after the removal of biofilm AS was synergy. According to the findings of this project, combined with previous studies, suggesting that the United clindamycin Galla chinensis extract for inhibition of bacterial biofilms has some viability, but the specific mechanism needs further study.