| Objection: In our present research we adapted nonbacterial chronic prostatitis model to research the expressions of PPARγ,NF-κB,IL-1βand TNF-αin the rat model of nonbacterial chronic prostatitis, and study the role of PPARγ,NF-κB,IL-1βand TNF-αin the rat model of nonbacterial chronic prostatitis.Method: 30 1-year-old male Sprague-Dawley rats were divided into two groups randomly, the normal group and the model group. The normal group received no treatment. The model group was castrated and injected estradiol benzoate (0.25 mg/kg/day, s.c.) for 30 days. After treatment for 30d,the pathologic appearance of the prostate tissue of the two groups were observed by HE staining and light microscope, and the expression of ppar-r in rat prostatic tissue was detected by means of RT-PCR.and the expression of NF-κB,IL-1βand TNF-αby means of irnmunohistochemistry(sp). Results: The control group did not have inflammatory reaction in their prostates,while the model group displayed visible inflammatory reaction. There is Negative expression of PPARγ,IL-1βand TNF-αin normal group and positive expression in the model group.and NF-κB is weakly positive in in normal group but strongly positive in the model group .The expression in normal group were lower than that of in model group(P<0.01).Conclusion: PPARγ,NF-κB,IL-1βand TNF-αplay an emportant role in the development of the nonbacterial chronic prostatitis and may be the new idear of pathophysiology and treatment for nonbacterial chronic prostatitis.PART 2 Protective effect of PPARγLigands(Pioglitazone)in the rat model for nonbacterial chronic prostatitisObjection: In our present research we adapted nonbacterial chronic prostatitis model to study the effection of Pioglitazone in nonbacterial chronic prostatitis,and to investigate the possible mechanism,provide a new method in clinical treatment. Method: 30 1-year-old male Sprague-Dawley rats were divided into three groups randomly, the normal group, the model group, the Pioglitazone group. The model group and the Pioglitazone group were castrated and injected estradiol benzoate (0.25 mg/kg/day, s.c.) for 30 days .At the same time the Pioglitazone group was given Pioglitazone 10mg/kg, one time a day,po,and others were given the same normal sodium,po.Until the 31's day, the pathologic appearance of the prostate tissue of the three groups were observed by HE staining and light microscope . the expression of PPAR-γin rat prostatic tissue was detected by means of RT-PCR.and the expression of NF-κB,IL-1βand TNF-αby means of irnmunohistochemistry(sp).Results: Control group did not have inflammatory reaction in their prostates.Model group displayed visible inflammatory reaction. Inflammatory reaction remitted obviously in the Pioglitazone group. There was no expression of IL-1β, TNF-αand weakly positive of NF-κB in normal group.Expression of them in the model group became significantly higher than that of the others group(P<0.01),But lower in the Pioglitazone group . PPAR-γmRNA was negative in control group but relative expression product was 0.63±0.13 in the model group and 0.25±0.10 in the Pioglitazone group,the difference had statistical significance. (P<0.05,n=10).Conclusion: PPARγmight have a role in the pathophysiology of nonbacterial chronic prostatitis. PPARγligands Pioglitazone could ameliorate nonbacterial chronic prostatitis,which may be associated with suppression the expression of NF-κB.PPARr ligands maybe a new treatment for the nonbacterial chronic prostatitis. |
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