The Research Of Establishing Intractable Epilepsy Model Of Phenobarbital-Resistant By Kindling The Rat's Amygdales

Objective: We created the temporal epileptic animal model through the chronic electrical stimulation in amygdale and got the refractory group through the administration of phenobarbital.Methods: Chose 85 four moon's age Wistar rats with the weight of 200g ad 250g, by randomization, choose five rats that was normal control group (group A), the rest used to seting up epilepsy animal model. Through the rat stereotaxis instrument, we inserted insulating nickel-chromium electrode to the two sides of rat's amygdales, consistented fixed, anti-infection by penicillin for five days,regained seven days after operation, electric-stimulused the amygdale (intensity: 500uA, wave space: 1ms, frequency: 16Hz, duration: 10s), the stimulus intensity was from 20uA, increased by degree-s 20uA eveytime, suscitation interval time was 1min, tracting the EEG by synchronization until the after discharge appeard, this current intensity was the pro-kindling ADT, after definiting the pro-kindling ADT for five min-utes, stimulated the amygdale repeatedly by 500uA until the Racine's IV–V behaviorappeard, this was kindling. When the kindling animal appeard 10 grand mals, twenty-four hours later, determined the meta-kindling ADT by the same method. When successfully seting up the epilepsy model, chose five rats that was physiological saline control group (group C), the restwere PB experimental group (group B) with injecting intraperitoneal by PB(30mg/kg), 1hour later, determined the ADT, as well as observing the drugadverse reaction, cut off the tail to get the blood, monitored the blood drug concentrations by high-performance liquid chromatography, monitored consecutively for three weeks. By the standard of group C'changes, we observed the group B'changes, we could diverge the non-responders-group (group D),responders-group (group E) and nonstationary-group (group F).Results:1. In all 80 rats, 42 rats were kindled, the sueessful kindling rate was 52.5%, through three drug screenings, in all 37 epilepsy rats, 8 rats were considered non-responders, the PB-resistant rate was 21.62%, 10 rats were responders, the non-resisitant rate was 27.02%.2. we can observe the behavior of Racine's IV–V.3. High-wave-spike and spontaneous seizures were observed.4. Blood drug concentrations did not differ significantly between responders and non-responders(P>0.05).Conclusion:1. we sueeessfully created ther refractory epileptic animal model, spo-ntaneous and iterative seizures were observed.2. The intractable epilepsy model of PB-resistant could simulate intractable complex partial seizures successfully, ADT was the sensitive index to judge the drug resistance.