| Objective: To study the model of development of PB-resistant amygdala-kindled rats.Methods: Chose 85 Wistar rats with the weight of 200g to 250g, by randomization, choose five rats to be normal control group (group A), the rest are used to setting up epilepsy animal model. Through the rat stereotaxis instrument, we inserted insulating nickel-chromium electrode to the right sides of rat's amygdales, consistented fixed, anti-infection by penicillin for five days, and after seven days recovery, electric-stimulused the amygdale. (intensity: 500uA, wave space: 1ms, frequency: 16Hz, duration: 10s), the stimulus intensity was from 20uA, increased by degree-s 20uA every time, suscitation interval was 1min, tracting the EEG by synchronization until the after discharge appeard, this current intensity was the pro-kindling ADT, after definite the pro-kindling ADT for five minutes, stimulated the amygdale repeatedly by 500uA until the Racine's IV–V behavior appeared, this was kindling. Twenty-four hours after the kindling animal appeard 10 grand mals, determined the meta-kindling ADT by the same method. When successfully seting up the epilepsy model, chose five rats that was physiological saline to be control group (group C), the rest were PB experimental group (group B) injected with intraperitoneal by PB(30mg/kg), 1hour later, determined the ADT, cut off the tail to get the blood, monitored the blood drug concentrate high-performance liquid chromatography, as well as observing the drug adverse reaction, every rat was given injection once a week and repeated three times. By the standard of group C'changes, we observed the group B'changes, we could diverge the non-responders-group (group D),responders-group (group E) and nonstationary-group (group F). A week after rats with different sensitivity to PB were selected, the rats of group D, E, A, C were decapitate to get their brain, immunohisto-chemistry method were used to determinate the expression of PGP in the brain of the rats, use two means of sample to test in order to analyze the result of ADT and PGP, and discuss the setting up of a model of intractable epilepsy as well as comment on the reliability.Results:1. In all 80 rats, 42 rats were kindled, the sueessful kindling rate was 52.5%, through three drug screenings, in all 37 epilepsy rats, 8 rats were considered non-responders, the PB-resistant rate was 21.62%, 10 rats were responders, the non-resisitant rate was 27.02%.2. We can observe the behavior of Racine's IV–V.3. High-wave-spike and spontaneous seizures were observed.4. ADT showed no remarkable increase in PB-resistant rats after injection of PB (P>0.05) , and PB-nonresistant rats exhibited remarkable increase in ADT(P<0.05) .5. P-glycoprotein showed statistically contrast in extensive area of bilateral cerebral hemispheres in the samples from PB-resistant rats as compared with which from PB-nonresistant rats . (P<0.05,0.01). Conclusion:1.We successfully created the refractory epileptic animal model.2. ADT was the sensitive index to judge the drug resistance.3.Using PB to screening kindling models to select PB-resistant amygalar-kindled rats would be feasible , it will be benefit the study of intractable epilepsy .
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