To Study The Effect Of Prevention And Mechanism Of Dracorhodin Perchlorate On Renal Injury In Diabetic Nephropathy Mice

Objective: To study the effect of prevention and mechanism of dracorhodin perchlorate on renal injury in diabetic nephropathy mice.Methods: 8 weeks old C57BL/6 male mice were randomly divided into six groups: normal control; diabetic nephropathy control; disease mice with administered by insulin; disease mice with administered by dracorhodin perchlorate at 20, 10, and 5㎎·㎏-1·d-1. Disease mice were induced by streptozotocin (STZ) 150㎎·㎏-1 peritoneal injection. The mice were sacrificed at the end of the 4th week. The metabolic data were measured at 4th week. The mRNA expression of Serum and glucocorticoid-inducible kinase 1 (SGK1), transforming growth factor-β1 (TGF-β1) and fibronectin (FN) was detected by RT-PCR. The expression and distribution of FN protein was examined by immunohistochemistry. Renal function was evaluated by creatinine clearance rate and proteinuria.Result: Dracorhodin perchlorate treatment and insulin treatment led to reductions of markers of fibrosis, creatinine clearance rate and proteinuria. The reduction is significant, dose-dependent in dracorhodin perchlorate treatment.Conclusion: Dracorhodin perchlorate can prevent and treat renal injury in diabetic nephropathy mice, and some mechanism may be related with the decrease of SGK1,TGF-β1 and FN in renal cortex.