Dracorhodin Perchlorate Can Inhibit High Glucose Active TGF-β1/SGK1/FN Signal Pathway In Human Mesangial Cells

Objective To investigate the effect of dracorhodin perchlorate(DP) on inhibiting high glucose-induced transforming growth factor-beta 1(TGF-β1)/serum and glucocorticoid induced protein kinase 1 (SGK1) / fibronectin (FN)signal pathway in human mesangial cells (HMC), and its mechanism of prevention and treatment on renal fibrosis in diabetic nephropathy (DN) .Methods The HMC were divided into Normal glucose group (NG group, 5.5 mmol·L-1D-glucose), Normal glucose +Low DP group (NG+LDP group,5.5 mmol·L-1 D- glucose+7.5μmol·L-1 DP),Normal glucose +High DP group (NG +HDP group,5.5mmol·L-1 D- glucose +15μmol·L-1 DP),High glucose group (HG group ,25 mmol·L-1D-glucose),High glucose +Low DP group(HG+ LDP group,25 mmol·L-1 D- glucose+ 7.5μmol·L-1 DP)and High glucose + High DP group (HG+ HDP group,25 mmol·L-1 D- glucose+ 15μmol·L-1 DP) . Each group was examined at 24hours. The level of TGF-β1,SGK1 and FN mRNA was detected by Reverse transcription-PCR,and real-time fluorescence quantitative PCR,and the expression of TGF-β1,SGK1 and FN protein was detected by Western blot and indirect immunofluorescence.Result A basal level of TGF-β1,SGK1 and FN in HMC were detected in NG group, and the level of TGF-β1,SGK1 and FN mRNA and protein were not evidently different compared to NG group by adding 7.5μmol·L-1 DP for 24 hours (P>0.05). On the other hand, the level of TGF-β1,SGK1 and FN mRNA and protein were obviously decreased by adding 15μmol·L-1 DP for 24 hour(sP<0.05or P<0.01); Compared to NG group, the level of TGF-β1,SGK1 and FN mRNA and protein were increased in HG group after stimulating for 24 hours (P<0. 01); Compared to HG group, the level of TGF-β1,SGK1 and FN mRNA and protein were evidently reduced in HG+LDP and HG+HDP groups by added 7.5μmol·L-1 DP or 15μmol·L-1 DP for 24 hours (P<0.05or P<0.01).Conclusion Dracorhodin perchlorate inhibit high glucose-induced transforming growth factor-beta 1(TGF-β1)/serum and glucocorticoid induced protein kinase 1 (SGK1) / fibronectin(FN)signal pathway in human mesangial cells, and this may be the part of mechanism to prevent and treat renal fibrosis of DN.