Research Of Syringa Leaves-Treatment Of Hepatitis New Drug

The medicinal situation has already been recorded in the "map of reality test" . Its leaves are the main part in medicinal. Syringa leaves were usually indications of acute dysentery, jaundice,hepatitis and fire eyes. Syringa leaves has been used in civil as "antibacterial anti-inflammatory, Qinggan Lidan" panacea. "Xinhua outline of Materia Medica" Indications records of Syringa leaves as "acute jaundice hepatitis, topical antimicrobial, the outbreak of fire eyes and swollen multiple drug Sores. In Civil, lilac leaf decoction used to treat eye and fire control violence is widespread dysentery. "Changbai Mountain Botanicals" said that Syringa leaves can "governance diarrhea, and hepatitis," .And the water of Syringa leaves can be drinked to realease hot, Syringa leaves extraction has strong inhibitory effect on Freund's Shigella.Our study is on the basic of the leaf of Oleaceae Syringa Syringa oblata L.as our raw material.We adopt Chinese medicine chemical research methods and combined with pharmacological model to selected the effective treatment of hepatitis type positions then the further reach on its extraction and separation and purification will be done to reach the requirements of effective site for of its content in Chinese medicine of five new drugs in more than 50% . Accordingly we developed a new type of anti-hepatitis Chinese medicine preparations not only for the Lilac of the medicinal resources development to provide important scientific value, but also Provide the basis for drugs for protecting Liver an cholecyst research.First of all, we study the ingredients for the Health and drug identification of Syringa leaves, to provide a scientific basis for the Syringa leaves as a valuable medical resources to further the health system pharmacy research and comprehensive development.Secondly, to screen the effective parts of Syringa leaves by the CCL4 liver damage caused by mice as a pharmacological model. Screening results showed that the extraction of the four groups in the extract that I and III are the effective locations, So we can based on this result to do our further purification of the study. In course of the study of effective part III, we adopted a uniform orthogonal experimental design process to determine the alcohol extraction methord,which is using 70% ethanol by returning extraction to get Alcohol-effective location III. Taking this as the basis for effective location for further purification of the study. By orthogonal experimental process to determine the alcohol extract methord, which is returning to 70% ethanol extract 2 times, each time one hour, for each increase of alcohol 13 times in order to further enhance the effective parts III content. then used absorbent absorption, by orthogonal experimental optimized absorbent purification process, that is 12 times the volume of ethanol extract of the dissolution, adding 1.5% of the adsorbent, minor stir 3 minutes, a total of 10 minutes adsorption In order get higher content of effective positions III. In the course of the study on effective part I , we have adopted uniform design orthogonal test to determine Treating water- boiled methord of the residue after alcohol extraction , that is, the water extract three times, each two hours, plus water, each 14 times; In order to reduce the impact of the greater polar impurities to increase the content of effective part I ,we use orthogonal experiment to optimize macroporous resin purification process, that is the first to three times the size water bed-eluting, and then to six times the size of the bed - 30% of ethanol cleared, the flow rate of two times per hour collection of medicines eluate, collecting 30% ethanol-eluting flow in, to get a higher content of the effective parts I . Through verification of the results show that effective water-soluble part I and Alcohol-effective part III as raw materials in the five categories of Chinese traditional medicine to achieve the requirements of new drugs which is more than 50%. Finally establish a stable, efficient, simple and economic separation from the process. According to the experimental results, after the preparation for the production and use of resources and lay the foundation for expansion.According to new drug research and development guidelines, we set up a pharmaceutical raw materials and preparation of quality standards, including characters, identification, inspection and determination. The goods per glycosides containing is not less than the total 137.5 mg; containing active ingredients A (C₁₇H₂₄O₉) should not be less than 6.25 mg. Stability and speed up the experimental results show that the stability of the goods in the storage period of stability.