| Objective: From the observation of the treatment of Warm Needle to rats which named Diabetes Peripheral Neuropathy Model, we can find the changes on their oxidative and oxidative resistance system. SO we will reveal the mechanism therapy of Warm Needle from protection of nerves. And we can provide the theoretical foundation of the treatment on this disease so to give play to the superior of Warm Needle.Method:(1)Choose 60 Wistar rats. Then randomly choose 10 of them into the normal group ,the rest are injected with STZ so that make them into the DM model rats. After 8 weeks, we divide these rats into model contrast group , Mecobalamin group and Warm Needle group.(2)Mecobalamin group is intramuscular injected mecobalamin everyday 300ug/kg,last 8 weeks; In the acqupuncturing group,acqupuncture the Gan Shu,Pi Shu,Shen Shu,Zu San Li,Huan Tiao,Yang Ling Quan while in the warm needling group ,hand some moxa on the needle and light it when acqupuncturing these point. Each point will have been treated 20 minites every other day,and totally 8 weeks.(3).Given a observation on the treatment effect on DPN by warm needling involved general situation,blood sugar,weight and nerve-transmit speed. The MDA was measured by Thiobarbituric fluorescence method .(4)Given a observation on the treatment effect on DPN by warm needling involved general situation,blood sugar,weight and nerve-transmit speed. The MDA was measured by Thiobarbituric fluorescence method and the SOD by Pyrogallol self-oxidize suppression.Results:(1)general conditions:During the experiment, except for normal control group, the diabetic rats in all groups developed obviously polydipsia, polyphagia, polyuria ,growth slowly and loss of body weight; the fur of diabetic rats are dirty and matte; spiritless, slowness of movement, accidie and extrados, etc.(2)the blood glucose level in warm needling group and acqupuncture group were lower than model group apparently (p<0.01) after 8 weeks and SNCV was faster than model group evidently (p<0.01) ,the SOD in blood serum and ischium nerve was higher than model group while the MDA was lower apparently ( p<0.01) ,the effects by warm needling were more obvious than acqupundture group (p<0.01) .(3)The changes of sciatic nerve conduction velocity:Compared with the normal-control group, the sciatic nerve conduction velocity of all treatment groups is significantly slow on the 16th week after treatment(P<0.05 ). the difference were significant(P<0.05),And at the same time there was no significant difference between methycobal-control group and acupuncture-treated group (P>0.05) .(4)The effects of electroacupuncture treatment on serum and sciatic nerve of MDA,SOD and TAOC:compared with the normal-control group, the rats of model-control group have a significantly increased in serum and sciatic nerve of MDA on 16th week after having been induced by STZ (P<0.01), but the content of SOD,TAOC in serum and sciatic nerve were obviously lower than that in the normal-control group in the corresponding time period (P<0.01). When compared with the model-control group the electroacupuncture treatment could attenuate the content of MDA in serum and sciatic nerve (P<0.05), and elevate the content of SOD,TAOC(p<0.05). These results had significant difference with methycobal-control group and acupuncture-treated group (P<0.05).Conclusion:(1)Warm needling could improve the symptom and sign of DPN rats .(2)Electro acupuncture treatment can protect the structure and function of peripheral nerves in diabetic rats is not by decreasing the blood glucose(3)Warm needling could make better the ability of resistant oxidation and inhibit the damage by active oxygen in the acts.
|
PDF Full Text Request