The Anti-HBV Mechanism Of Herbae Artemisiae Capillariae Modified Decoction In In Vivo And In Vitro

Virus hepatitis has emerged as a major public health problem throughout the world affecting several hundreds of millions of people.At present,five known hepatitis viruses,belonging to respective viral families,directly affecting the liver and causing the different types of viral hepatitis,have been discovered.There are hepatitis A,B,C,D,E virus.Hepatitis B virus is the pathogen of hepatitis B.Hepatitis B virus(HBV),together with woodchuck hepatitis virus(WHV),ground squirrel hepatitis virus(GSH),duck hepatitis virus(DHBV) belong to the Hepadnaviriade family.And it is a 42-nm spherical particle,comprising the nucleocapsid with 27nm in diameter surrounded by an outer envelope of the surface protein(HBsAg) embedded in membranous lipid derived from the host cell.The nucleocapsid of the virion consists of the viral genome surrounded by the core antigen(HBcAg).HBV replicates within infected hepatocytes,it is the most important agent responsible for parenterally transmitted viral hepatitis.The pathology is mainly mediated by the responses of the cellular immune response of the host to the infected hepatocytes.My essay is based on the study of modern medicine an tradional Chinese medicine.In order to invest the anti-HBV mechanism of Herbae Artemisiae Capillariae Modified Decoction on DHBV in vivo and in vitro on HepG2 2.2.15 cell.Herbae Artemisiae Capillariae Decoction comes from "Shanghan Lun",is composed of artemisiae capillaris,gardenia and rhubarb,the artemisiae capillaries is principal drug,it can Clear away heat and promote diuresis; the gardenia is ministerial drug,can Tongli tripie warmer,lead humid heat to out through urine;rhubarb is adjuncticve drug,can eliminate heat and stasis.They three together can clear away heat and promote diuresis,to neutralize poison and eliminate aurigo.Mainly used for treat humid heat aurigo.Modern research suggest that they can cholagogue,protect Liver,relieve fever,anti-inflammatory,relieve pain,antibiosis and antiviral.The study in vivo suggest that OD490 value of serum DHBV-DNA of large dose Herbae Artemisiae Capillariae Modified Decoction group was significantly decreased on the 21^st day and 28^th day after treatment than that before treatment (P＜0.01,P＜0,05);The lamivudine group was significantly decreased on the 14^th day,21^st day and 28^th day after treatment than that before treatment and that of model group(P＜0.01,P＜0,05).The research in vitro found that the cell's growth cycle is about ten days.Its' secretion of HBsAg and HBeAg were well,and its' peak was the 4^th or 5^th day,but its' secretion of DNA was low;The cytotoxicity experiment of drug suggest that The TC₅₀(median toxic concentration) of Herbae Artemisiae Capillariae Modified Decoction on HepG2.2.15 is 26.16 mg/ml.The TC₅₀ of 3TC over 1mg/ml.And the IC50 of Herbae Artemisiae Capillariae Modified Decoction to HBsAg is 13.1mg/ml,the TI is 2.00,to HBeAg is 18.4mg/ml,the TI is 1.42.The IC50 of 3TC to HBsAg is 0.48mg/ml and the TI＞2.08.