Research Of Oxidation Function Indexes Changes In Workers Exposure To Toluene, Methanol, And Dimethylformamide For A Long Time

Background:The high exposures of the human body to poisonous chemicals can result in the intoxication.The representation of poisonous effects of chemical materials includes stimulus,hypersensitiveness,hypoxia,coma,anesthesia,all over the body intoxication, carcinogenesis,malformation and mutation.The different poisonous chemical materials can induce damage in different genealogy including nervous system damage,acute respiratory system damage,alimentary system damage,urinary system damage and circulation system damage,even multiple organ damage.Some damage in hematological system is the most form.For example,benzene,arsenicum,and lead can lead the anaemia;benzene,glossy-acetic acid etc can lead to granulocytopenia; amid-benzene and nitro-benzene can damage the erythrocyte and induce hemolysis; benzene,trinitrotoluene,arsenicum compound,carbon tetrachloride can depress the function of haematogenesis and increase the akaryocyte,leucocyte and thrombocyte to initiate the aplastic anemia.As we known that benzene can induced the leukemia with the morbility 0.14%o.Toluene,methanol,and dimethylformamide were heavy used in industry as organic solvent in Zhejiang Province.Especially in the accessory ingredient industry,more and more workers use them.It is necessary to research the changes of GSH,SOD,NO,MDA and PON1 in workers exposure to low concentration of toluene, Methanol and Dimethylformamide for a long time,for workers in that environment usually have no overt symptom.Toluene can induce the metabolite of hydroxide radical free radical after it come in the body.Free radical is the most vivid vigor oxygen which can react with saccharum,phospholipid,and nucleotide to damage the cell.When it reacted with the cell membrane,it can lead the cellular membrane lipid peroxidation, dysfunction of protein,enzyme and DNA,and ultimately lead to the change of oxidation dysfunction,metabolic disorder,damage of DNA and cell dedth.The feature of antioxygen system is that increase of antioxidase and synthesis of some antioxidant.It come a hot spot to research the changes of antioxygen system as a biological tag in ecotoxicology subjects.We explore the changes of GSH,SOD,NO,MDA and PON1 in peoples getting in touch with low concentration of Toluene,Methanol and Dimethylformamide for a long time,and to find the mechanism of effect of antioxygen system in the chronic intoxication.Objective:To explore the changes of GSH,SOD,NO,MDA and PON1 in peoples exposure to low concentration of Toluene,Methanol and Dimethylformamide for a long time. Methods One hundred and sixty-two workers exposure to Toluene,Methanol and Dimethylformamide for a long time were divided into three groups:Toluene group (n=28,group A),Methanol group(n=41,group B) and Dimethylformamide group(n=43,group C).The control group(n=50,group D) was also chosen.All workers were drawn off the blood samples to detect the GSH,SOD,NO,MDA,ChE,and PON1. Results:NO changes:compared with the group D,NO significantly increased(P＜0.05) in other groups;NO is higher in group C than that in group A,P＜0.05,Compared with group D,SOD significantly increased in the other groups(P＜0.05 or P＜0.01).SOD was lower in group A than that in group C(P＜0.05),and slower in group B than that in group C(P＜0.01).Compared with group D,GSH significantly decreased in other groups(P＜0.01),but there was not significant difference in other group(P＞0.05). Compared with group D,MDA only significantly increased in group B(P＜0.05),there was not significant difference in other groups.Compared with group D,PON1 significantly decreased in other groups(P＜0.01),but there was not significant difference in other groups(P＞0.05).Conclusions:After long time getting in touch with Toluene,Methanol and Dimethylformamide, which even is lower than National Standard of Toluene,Methanol and Dimethylformamide,workers' oxidation function significantly changed,although these peoples had not visible symptom.