Studies On The Chemical Constituents And Antitumor Activity Of Forsythia Suspense (Thunb.) Vahl.

Phillyrin as Oleaceae plants Forsythia(Forsythia suspense(Thunb.) Vahl.) Dry ripe fruit,is one of Chinese clinical commonly used tradition traditional Chinese medicines,has the clearing heat and detoxication,relieves congestion effect of the detumescent,relieves congestion effect of the detumescent,mainly treats warming up,the erysipelas,macula,the ulcer to swell the poison,scrofula, the urination to drench shuts and so on sickness.The ethanol extract of Forsythia are commonly used anti-cancer drugs to one group,clinical put on regular Phillyrin formula made with other Chinese herbal medicine,treatment of head and neck tumor, esophagus,stomach,breast,cervical cancer,leukemia and so many kinds of tumor.Had the literature report forsythia ethyl alcohol extraction to have broad-spectrum anti-tumor activity has on a variety of tumor cells significantly inhibited.In order to explore its anti-tumor active material foundation,this experiment in the earlier period research's foundation,uses the active strongest ethyl acetate part which the system solvent extraction screens to carry on the separation,uses the chromatograph parting method to separate 12 monomer compound from this active site,and has carried on the structure appraisal with chemistry and spectrum method these kind of compound,respectively be Betulinic acid, Oleanolic acid,ursolic acid,2α,23-OH,ursolic acid,phillyrin,phillygenin,epipinoresinol,isolarlciresinol,daucosterol,β-Amyrinacetate.,β-sitosterol,quercetin.The separation and identification three epoxy lignin monomer compound,observes these 3 monomer compound through the MTT colorimetric to human gastric cancer cell line SCG7901 growth inhibitory action discovery,the phillyrin and the epipinoresinol has cerin inhibitory action to human gastric cancer cell line SCG7901 growth,but the philiyrin 's inhibitory action is not obvious.The above findings were specially the epoxy lignin class material further deep research have laid the foundation for the weeping forsythia active material.