Study On The Chemical Constituents And Bioactivities Of The Bark Of Ulmus Pumila L.

Our country is rich in resources of Chinese herbal medicine,the theory of Chinese medicine is extensive and profound,and a lot of experience has been accumulated in the prevention and treatment of tumors.As Chinese herbal medicine has a mild and long-lasting effect and small toxic and side effects,it is necessary to find effective parts or monomer compounds with better anti-tumor activity and develop it into a new anti-tumor drug with good curative effect and high safety.It is suitable for my country’s national conditions and has broad development prospects..Through literature research in the early stage,the research team found that most of the types of Chinese medicines used to treat tumors are heat-clearing and detoxifying,anti-inflammatory and swelling,promoting blood circulation and removing blood stasis,etc.,and initially screened 18 Chinese medicinal materials that may have anti-tumor activity,and conducted preliminary studies.The activity screening of Ulmus pumila L showed that Ulmus pumila L has certain anti-tumor activity.Ulmus pumila L,also known as elm white bark,is the phloem part of the bark and root bark of the Ulmaceae plant（Ulmus pumila L.）.It is widely distributed in various provinces and regions in China and has abundant resources.As a traditional Chinese medicine in my country,it is often used to soothe the nerves and invigorate the spleen,relieve water and relieve leaching,and relieve phlegm and cough.External use can also treat bone fractures,outer mountains,bleeding,etc.It also has a certain effect on frequent burns and scalds.Modern pharmacological studies have shown that Ulmus pumila L has various pharmacological activities such as anti-inflammatory,anti-oxidant,antibacterial,anti-septicemia,etc.,especially in anti-tumor,which has more potential research value。At present,there are few reports on the chemical composition of Ulmus pumila L,and the research on its chemical composition is relatively weak.In order to further enrich the research data of the traditional Chinese medicine Ulmus pumila L,this paper conducts a systematic research on the chemical composition of Ulmus pumila L,and evaluates the anti-tumor activity of the obtained monomer compounds in vitro.Extract 50 kg of Ulmus pumila L with an 80%ethanol solution,and then extract by parts to obtain about 450 g of petroleum ether,500 g of ethyl acetate,and 800 g of n-butanol.Separate and purify ethyl acetate and n-butanol.Use ODS column chromatography,silica gel column chromatography,Sephadex LH-20 column chromatography,recrystallization and other methods for separation and purification,and then combine the physical and chemical properties of the obtained monomer compounds and use ESI-MS,1H-NMR,13C-NMR,etc.The structure of the obtained compound was identified by spectroscopy technology.A total of 22 compounds were obtained,namely,Betulinic acid（1）,Cleomiscosin C（2）,Friedelin（3）,Ursolic acid（4）,6-Hydroxyrubiadin（5）,1,3-Dihydroxyanthraquinone（6）,7-Hydroxy-6-methoxycoumarin（7）,3-Hydroxy-4-methoxybenzoicacid（7）,3-Hydroxy-4-methoxybenzoicacid（8）,22-O-（4-hydroxy-3-methoxy-cinnamyl）-docosanoic acid（9）,Mansonone E（10）,Mansonone H（11）,β-sitosterol（12）,kaempferol-6-C-β-D-glucopyranoside（13）,Lyoniside（14）,Isohemiphloin（15）,6-C-glucosylquercetin（16）,Fraxin（17）,Catechin（18）,Schizandriside（19）,Scopolin（20）,Catechin-7-O-β-apiofuranoside（21）,daucosterol（22）.Among them,compound 3 and compound 9 were isolated from this plant for the first time.The MTT method was used to evaluate the anti-tumor activity of the obtained monomer compounds in vitro.The lung cancer cell line H460,the melanoma cell line B16F10,the cervical cancer cell line Hela,and the breast cancer cell line MCF-7 were selected to detect the compounds isolated from Ulmus pumila L.The screening results of antitumor activity in vitro showed that the half inhibitory concentration(IC₅₀)of compounds 10,11for cancer cells H460 were 6.84±0.09,30.19±0.64 ug/ml,for cancer cells MCF-7 were0.043±0.001,18.55±2.45ug/ml,for cancer cells Hela were 7.26±0.29,21.94±4.39 ug/ml and for cancer cells B16F10 were 7.05±0.09,29.14±7.89 ug/ml.