Study On The Pharmacognosy Of Euphorbia Ebracteolata Hayata. In Shandong

Objective: To study the pharmacognosy of Euphorbia ebracteolata Hayata.from the plant morphology, bionomics, characteristic of the medicinal material, interior structure, quality guideline and chemical compounds, exploit and utilize the resource of the Euphorbia ebracteolata Hayata., provid the scientific basis for establishing the standards of quality guideline.Methods: Adopting the routine methods to survey the plant morphology, bionomics and the characteristic of the medicinal material; and utilized the microscopical observation to study the histological structure and powder feature of Euphorbia ebracteolata Hayata., research the TLC, ash, moisture and extraction by the methods supplied in the Chinese Pharmacopoeia; The compounds were isolated and purified by repeaded normal column chromatography and sephadex chromatography, and the structures were elucidated by chemical evidence and spectral methods; In addition, the volatile components of the roots of Euphorbia ebracteolata Hayata.was carried out by the GC-MS.Results: Through the research of the pharmacognosy of Euphorbia ebracteolata Hayata., summarize the key character of this species and the main characteristic of microscopic identification, such as the compound vascular bundle, the yellow laticiferous tube and considerable starch grain. Take theβ-sitosterol,β-amyrin acetate,Jolkinolide B and 2,4-dihydroxy-6-methoxy-3-methyl-acetophenone as the reference substance to determine the samples of the Euphorbia ebracteolata Hayata., and the same spans can be seen on the TLC charomatogram, so the anatomic structures and TLC can be used to identify Euphorbia ebracteolata Hayata..By the studying of the quality guideline of the Euphorbia ebracteolata Hayata.,the content of security moisture do not exceed 10%, the content of total ash is 6.58%, the content of total ash which do not dissolve in the chlorhydric acid is 1.90%, the content of the aqueous extractum and alcohol extractum are respectively 23.26%,14.88%. Also, ten compounds were isolated,which were identified asβ-amyrin acetate(EE-1),jolkinolide A(EE-2),JolkinolideB(EE-3),β-sitosterol(EE-4),2,4-dihydroxy-3-formyl-6-methoxy-acetophenone(EE-5),2,4-dihydroxy-6-methoxy-3-methyl–acetophenone(EE-6),scopoletion(EE-8),3,3/-diacetyl-4,4/-dimethoxy-2,2/,6,6/-tetrahydroxydiphenylmethane(EE-7),Tridecylferulate(EE-9),and2-hydroxy-6-methoxy-3-methyl-acetophenone-4-O-β-D-glucopyranoside(EE-10). In addition, twenty-nine compouds were identified.from the the volatile components of the roots of Euphorbia ebracteolata Hayata.by GC-MS.Conclusion: The Euphorbia .ebracteolata Hayata. has the manifest feature of the plant morphology and bionomics, also has the the typical identification characteristics of the medicinal material and microstructure, meanwhile, it has the special structure of the chemical constituents. In this paper, ten compounds were isolated and identified, in which two compounds were isolated for the first time, and twenty-nine compouds were identified from the volatile oil. Also, study the quality of medical material of Euphorbia ebracteolata Hayata., From the researching, enriche this species researching content, laid a foundation for further development of this species.