| Objective: To investigate the therapeutic effect of four species of extracts and active components from Vitex negundo seed on human ovarian cancer CoC1 cell line.Methods:The viability of CoC1 cells was determinated by MTT assay; The trypan blue exclusion method was used to investigate the growth inhibitory effect of EVn-50 and four species of Lignans of Vitex negundo seed in CoC1 cells.The established tumor xenograft in nude mouse model was used to evaluate therapeutic effect of EVn-50 and four species of Lignans on human ovarian cancer. SP immunohistochemical method was used to analysis expression of vascular endothelial growth factor (VEGF) , proliferating cell nuclear antigen (PCNA) and Platelet endothelial cell adhesion molecule-1(PECAM-1,CD31) in human ovarian cancer xenograft.Results: Four species of extracts of Vitex negundo seed all inhibited viability of CoC1 cells. The acetoacetate extract of Vitex negundo seed(EVn-50) was the strongest in those,and it's IC50 value was 1.02μg/mL. EVn-50 and 4 species Lignans of Vitex negundo seed had growth inhibitory effect on CoC1 cells, Comp 8 was the strongest in those, with IC50 value was 1.40μg/mL, which similar to EVn-50 (IC50 was 1.56μg/mL).The tumor growth inhibitory of EVn-50 and Comp 6 were 57.1%, 55.7% respectively, and the tumor weight inhibitory rates were 61.6 % , 51.5 % respectively. Comp 8 had little effect. Expression of PCNA , VEGF and CD31 protein in human ovarian cancer xenografts all were down-regulated.Conclusion:1. The acetoacetate extract of Vitex negundo seed(EVn-50) can inhibit viability and growth of human ovarian cancer CoC1 cell line.2. The Lignans from the acetoacetate extract of Vitex negundo seed have an inhibiting effect on growth of human ovarian cancer CoC1 cell line.3. The acetoacetate extract and Comp 6 can inhibit the growth of human ovarian cancer CoC1 tumor xenograft in nude mice, and the mechanism may be correlated with the down-regulation of VEGF , PCNA and CD31 protein.
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