Chemical Constituents Of Celastrus Orbiculatus Thunb. And Technology For Extraction Of Pristimerin From This Plant

This thesis was consisted of three parts. The extraction and separation of chemical constituents from root bark of Celastrus orbiculatus Thunb, the technology for extraction of pristimerin from this plant and the review on advancesin proteasome inhibitors, were reported respectively. Ten compounds were isolated from the root bark of C. orbiculatus in the first part, and eight of them were identified including two quinonemethide triterpenes. The technology for extraction of pristimerin, the main active constituent of C. orbiculatus, was discussed in the second part. The third part was mainly about the advances in proteasome inhibitors in recent years.In the first part, the experiments of isolation and identification had been done by using modern technology. Ten compounds were isolated from the rootbark of C. orbiculatus, and eight of them were identified as 3, 22-dioxo-20-ta-raxastene (1), sugiol(2), pristimerin (3),β-sitosterol (4), celastrol (5), salapermicacid(6), benzoic acid (7), daucosterol (8) on the base of physical, chemical andspectral data.Compound 3 and 5, belonging to quinonemethide triterpenes, werereported to be potent proteasome inhibitors.In the second part, a proposal about the main influence factors of technol-ogy for extraction of pristimerin was made on the basis of modern statistics methods. And the content of pristimerin was determined by HPLC. Finally a method of two steps of column chromatography was obtained.The third part is an overview about study of proteasome inhibitors in rece-nt years. The development of natural proteasome inhibitors was underlined. It will be helpful in the following study of proteasome inhibitors.