Study On Protecting Effects Of Lipsomal Clodronate On Renal Injury Acute Pancreatitis

AIM Acute necrotizing pancreatitis(ANP) regarding as a kind of severe acute abdominal diseases in surgery are often complicated by multiple organ dysfunction syndrome(MODS). With the deep study on the pathogenesis of ANP mechanism, it is demonstrated that macrophages play an important role in the development of ANP .This paper is to investigate the protective effects of liposomal clodronate on renal injury in rats with ANP and the assessment of severity of renal injury.Method Totally 48 rats were randomly divided into three group: normal control group (C); ANP group, in which rats were treated with pure liposomal (P) ; treatment group,in which rats were treated with liposomal clodronate disodium (T). The ANP model of rat was induced by injection of 5% sodium taurocholate beneath the pancreatic membrane . Rats of normal control group received isovolumetric injections of 0.9% physiological saline solution instead of sodium taurocholate . Blood samples were collceted to measure AMS, BUN, Cr, IL-6 and IL-12 at 2 hours and 6 hours after ANP. At the same time, pathologic alteration of pancreas and kidney were observed and the pathological score of pancreatic and renal tissue were also estimated. Results Compared with control group, serious renal and pancreatic damages were found in group P, and the pathological score of pancreatic renal tissue were elevated significantly (P< 0.01 ), and the AMS, BUN, Cr levels were elevated significantly (P< 0.01) .Compared with group P, the renal and pancreatic damages were attenuated in group T , and the pathological score of pancreatic and renal tissue were decreased significantly(P< 0.01), and the levels of Cr and AMS were decreased significantly (P< 0.01 ), and the IL-6, IL-12 were decreased at 2 hours and 6 hours (P< 0.01). The BUN was decreased significantly at 6 hours (P<0.05) . Conclusions Excessive release of inflammatory mediators play an important role in renal injury in ANP. Liposomal clodronate disodium can alleviate the damage of pancreas and kidney.