Chemical Constituents From Two Species Of Smilax. And Their Antitumor Activities

There are more than 60 species of Smilax in China. Many steroidal saponins separated from the genus and some of them show certain biological activities till now. Herein, The constituents of the rhizomes of Smilax. China L. and Smilax riparia DC. were isolated and purified by repeated silica gel chromatography, SephadexLH-20 and macroporous resin. Six compounds were obtained and four of them were identified as apigenin-7-O-rhacoside(c), 2-methyl-5-(1,1-dimethoxyl-l-formyl) furan(2), ripariaol(3) on the basis of MS, 1D and 2D NMR spectroscopic analyses and chemical evidence. Compounds 1,2 were separated from S. china. L and the other one was separated from S. riparia DC.. Compounds 2,3 are reported for the first time,expecially Compounds 3 is a compeletely novle structure,and Compounds 1 were isolated from the genus Sm ilax for the first time.Some pharmacological technologies were used to study the antitumor activity of extracts towards melanoma cells B16 and breast cancer cells MCF-7 in vitro. Extract A, G inhibited the growth of B16 cell best, their IC50 are 2μg/mL,30μg/mL respectively. The results suggested that extract A, G play important roles in the antitumor activities of S. china L. and S. riparia DC. However, all extracts of S. riparia DC. inhibited the growth of the breast cancer cells MCF-7 effectively especially at their highest concentration (1,000μg-mL-1). The result indicated that S. riparia DC. have certain bioactivities against breast cancer.The structural elucidation of some complex compounds and the anti-tumor re-screening of some phenolic constituents and extracts from S. china L. are carrying out on the way now.