Determination Of Trace Element In Blood Serum And The Analysis Of Correlative Factors In Patients With CHD

Background and objective: Coronary atherosclerotic heart disease refers to lumina stenosis or obstruction resulted from coronary atherosclerosis which leading to myocardial, ischemia, hypoxia. It Referres to coronary heart disease with high incidence, high mortality,and high morbidity. In recent years, a lot of researches have found that the incidence and development of cardiovascular disease is closely related with trace element imbalance or metabolic disorder. Trace elements play an important role in the construction and relaxation of myocardium, cell membrane structure and function,lipid metabolism and stability, the catalysis and blanket of free radicals as well as blood pressure accommodation and blood coagulation. The study through measuring serum levels of Zn, Cu, Fe content, to initial serum Zn, Cu, Fe level and the relationship between coronary heart disease-related factors, in order to provide the theoretical basis and practical accordance to the diagnosis and prevention of coronary heart disease.Methods: We chose 117 patients with coronary heart disease from all of the patients who treated in the Cardiovescular department and clinic of the first hospital of Jilin University From January 2009 ~ February 2010 in random.There are 52 patients with AMI (A group), 65 patients with coronary heart disease but without AMI, (B group), and 60 cases of healthy subjects without coronary heart disease as the control group (C group). All selected patients were gatherd for 4ml ulnar vein blood in the second morning admitted to hospital with an empty stomach (>8 hours, <24-hour after admitted). The blood was putted in a test tube without anticoagulant and separated by centrifugation immediately in the speed of 3000 rpm/min to centrifugal 15 min. Then draw the upper pipette to a 2ml tube of serum, and the tubes were sealed with film sealed, and then the specimens were saved at -80℃refrigerator. All specimens were sent to laboratory within a month,to trace the level of copper (Cu), zinc (Zn), iron (Fe), magnesium (Mg) By the U.S. Beckman LX20 automatic biochemical analyzer .Analyzer Results: (1) The average age in group A is 57.65±13.0 years ,the proportion of male patient is 73.08%, the proportion of type 2 diabetes is 36.54%, the proportion of hypertension is 32.69%, proportion of hyperlipidemia is 44.23%, and the smoking rate is 51.92%. The average age in group B is 61.12±10.29 years, the proportion of male patient is 69.23%, the proportion of type 2 diabetes is 35.38%, the proportion of hypertension is 32.31%, proportion of hyperlipidemia is 44.62%, and the smoking rate is 50.77%. The average age in group C is 58.48±10.82 years, the proportion of male patient is 73.30%, the proportion of type 2 diabetes is 11.67%, the proportion of hypertension is 18.33%, proportion of hyperlipidemia is 25.00%, and the smoking rate is 33.33%. There was no statistically significance through A, B and C groups in age, sex ratio and incidence of hypertension.The Smoking rates, incidence of hyperlipidemia and the incidence of type 2 diabetes of group A and B were higher than C group (P<0.05), there was no significant difference between group A and B (P > 0.05). (2) The results show that levels of serum Zn of the group AMI are lower than Control group (P<0.05), levels of serum Zn of the group with CHD but without AMI are lower than Control group (P<0.05), levels of serum Zn of the group AMI are lower than group with CHD but without AMI (P<0.05), levels of serum Cu of the group AMI are lower than Control group (P<0.05), levels of serum Cu of the group with CHD but without AMI are lower than Control group (P<0.05), the levels of serum Fe was no significant difference between group AMI and the group with CHD but without AMI (P >0.05), levels of serum Fe of the group AMI are higher than Control group (P<0.05), the levels of serum Fe was no significant difference through group AMI and group with CHD but without AMI, the levels of serum Mg was no significant difference through of the groups. (3) There was no correlation between the Zn, Cu, Fe levels and the coronary artery disease count (P>0.05). (4).The level of Serum Zn and coronary have linear correlation trend with Gensini integration. The level of Zn has negative correlation with coronary artery through Gensini integration (r=-0.7310, P<0.05). Gensini scores were not associated with the level of Cu (r= -0.1156, P>0.05). Gensini scores were not associated with the level of Fe (r= -0.1668, P>0.05).(5) Factorial analysis was used to define the affect of diabetes and the use of statins to the levels of Zn,Cu and Fe,and test the interaction between factors.The study discovers that the interaction between diabetes and the use of statins has no effect on he levels of Zn and Cu(P >0.05). But the interaction between diabetes and the use of statins has great effect on he levels of Fe(P=0.038).Conclusions: (1) The level of Zn, Cu and Fe have relationship with coronary atherosclerotic heart disease. (2) The negative relationship between Zn levels in patients with AMI and coronary artery Gensini Integral Can be used as evaluation of coronary artery disease severity in patients with secondary targets. (3) The interaction between diabetes and the use of statins has great effect on he levels of Fe in patients with Coronary atherosclerotic heart disease.