The Effect Of Drug-Containing Serum Of Slr On Cell Viability Of Huvec Oxidative Damage Model By H₂O₂ Induced

Objective: To observe different concentration drug-containing serum of SLR that is one ministerial drug of recuperating-heart decoction effect the HUVEC oxidative damage model by H2O2induced, to investigate the potential mechanism of to action about it to research mechanism of to action about recuperating-heart decoction for prevented and cured UA(unstable angina), to establish fundament for new drugs to prevent and cure UA.Method: To apply prepared method of drug-containing serum, the 30Wistar rabbits are divided into two groups randomly that blank group and SLR group. the rabbits are gived SLR decoction in the SLR group, the rabbits are gived isodose physiological saline in the blank group. intragastric administration is 2 times a day and continued a week. blood collection is from heart of rabbits at 1 hour latter of the last administer. Then to separate blood serum and to prepare drug-containing serum culture fluid. To apply cell culture method, the refrigerant saved HUVEC is revived, cultivated and passed generation. The cells were digested, collected and counted until the cell condition stabilized. The cell suspension were transfered in culture plank with 96 hole, there are every2×104cells in every hole. It was divided five groups. They are H2O2 model group, blank blood serum group, 2% drug-containing serum of SLR group, 4% drug-containing serum of SLR group, 6% drug-containing serum of SLR group. There are 6 annexal hole in every group. It is interfered in drug-containing serum with different concentration for 24hours in every drug-containing serum of SLR group. It is cultivated with common culture solution for 24hour in H₂O₂ model group and blank blood serum group. then HUVEC is interfered in 100μmol/L H₂O₂ for 2 hours(except blank blood serum group).to copy the Atherosclerosis cells model. morphological variation is observed by inversion flourescence digital imaging microscope. The cells viability is measured by MTT assay. Result :1,morphological variation: the cells growth well appearing typical slabstone in the blank blood serum group. the cells damage obviously in the H2O2 model group.while in groups of drug-containing serum of SLR ,condition of the cell is improved and depend on concentration of drug-containing serum of SLR.2,the result of MTT indicated that the OD value of HUVEC decreased obviously in the H2O2 model group. comparing with blank blood serum group, it was considered statistically significant (p<0.01) The OD value of HUVEC in 2%,4%,6% drug-containing serum of SLR group increased obviously comparing with the H2O2 model group, it was considered statistically significant (p<0.01).to compare with each other during every drug-containing serum of SLR group, it was considered statistically significant (p<0.01).there is concentration dependent..2%,4%,6% drug-containing serum of SLR group can increase the growth rate of HUVEC by H2O2 damage, comparing with the H2O2 model group, it was considered statistically significant (p<0.01). to compare with each other during every drug-containing serum of SLR group, it was considered statistically significant (p<0.01). there is concentration dependent.Conclusion: The drug-containing serum of SLR that is one ministerial drug of recuperating-heart decoction can protect HUVEC by H2O2 damage. It can improve morphological variation of HUVEC by H2O2 damage, advance the enhancement of damaged endothelial cell, increase the viability of damaged endothelial cell by antioxidative effection. This is maybe the one of mechanism of action for prevented and cured UA.