Expression Of TIMP-1 And MMP-13 In Rabbit Articular Cartilage Of Knee Joint Or Expression Of TNF-α And IL-1β In Synovial To Heavy Metal Lead Exposure

Object:1. To study the blood, bone and articular cartilage and synovial of lead content in the rabbits which was drinked lead pollution of water2. To study the mRNA of TIMP-1 and MMP-13 in the knee joint articular cartilage and the mRNA of TNF-αand IL-1βin the synovial in response to lead pollution of water.Method:At the age of 2.5 months,34 New Zealand rabbits(1.3-1.5Kg) which were divided into 2 parts started drinking lead pollution of water and pure water.The water of concentration which was polluted by lead is 1.0 g/L.Thereafter,the rabbits were raised for 3 months.Then,they will be executed by ear margin veins air embolism, take its blood,bone, cartilage and synovial respectively,and test lead content by machine.At the same time, about 200mg knee cartilage will be restore in tube and frozen in the liquid nitrogen cryopreservation.we extracte the Mrna and measue the mRNA expressions in the knee cartilage and synovial with RT-PCR..Result:1. The control group and lead exposure group's blood, bone, cartilage and synovial all can be detected , but two groups of heavy metal lead with bones in both the highest levels, which in turn for synovial, cartilage and blood. Compared with control group, the amount of lead in the group of ead exposure were significantly increased in the blood, bone, cartilage and synovial(P < 0.05).2. Compared with control group,the mRNA of TIMP-1 is significantly reduced in the lead exposure group,but the mRNA of MMP-13 is significantly increased.and the mRNA of TNF-αand IL-1βin the synovial is significantly increased(P < 0.05).Conclusion:When the rabbits was drinked for 3 months,we can measure the lead in the blood, bone, knee cartilage and synovial,and it can damage the cartilage and synovial significantly.