Cigarette Smoke On Male Rats Hormones And Seminal Plasma Biochemical Impact Study

Background:Infertility is a global medical and sociology important problem, is to influence both sides of husband and wife and family harmony of important factor. The world health organization regulation, couples cohabiting over one year, without using any contraceptives, because the man factors is the male sterility sweetheart infertility. Male infertility is not an independent disease, but many diseases or the result of many factors. The major factors that affect the male sterility are:①Sperm abnormalities;②Testicular abnormal;③Endocrine factors;④Genetic factors;⑤Immune factors;⑥Reproductive organs infection;⑦Environmental impact etc.Smoking is common in people life a bad living habits, growth is the main crowd male smoking. According to statistics, global thirteen million smokers, including nine million people in developing countries, China has reached 3.2 million smokers. The world health organization says tobacco use and death and the growing global epidemic disease spread highly correlated. The smoke cigarette burning main toxic compounds including carbon monoxide, nicotine and tar, these substances through breath enter human body can cause multi-system damage. Cigarettes, complicated composition containing 4,700 compounds, including more than 3000 kinds have been recognized in cigarette smoke and has proved with many substances to cause cancer, birth defects and mutation role.Smoking on people with various influence body, it can cause high blood pressure, coronary heart disease, stroke, cancer and other diseases. Cigarette smoke on the male reproductive system influence mechanism is unclear. Cigarette smoke exposure to the influence of serum study of domestic and foreign literature Associated Press, but the result has different; The influence of seminal plasma biochemical research reports for less. Cigarette smoke exposure on male rats and seminal plasma biochemical joint research did not see related reports.Purpose:1.Research on male rats cigarette smoke the influence of sex hormones (testosterone,Follicle-stimulating hormone,luteinizing hormone)2. Research on male rats cigarette smoke the influence of pure pulp biochemistry (fructose,zinc)Methods:1.August 30 ZhouLing only by weight of male SD rats were randomly divided into smoking group, quit smoking group, the control group, each group of each 10 only.2. Prior to the content of each unified picks detection sex hormones.3. Smoking only with the control group of five only 10, after eight weeks, blood testing sex hormones content Dissection of seminal fluid and take the prostatic secretion of pure berries sugar, detection zinc content; Take the left testis line profile HE dyed. Testicles. Stop smoking group only with the control group 5 only 10,12 weeks according to the above methods related inspection.4.Adopt radioimmunoassay detection serum follicle-stimulating hormone, testosterone, eery corpus luteum content; Using uv-colorimetry method in detecting seminal plasma sugar contents; By colorimetric method to detect seminal plasma zinc content.Results:1.Sex hormones detection results:experiment measured before each group T, FSH and LH, content are there were no obvious difference. Smoking group T, FSH, LH content before were lower than that of the experimental group are smoking and differences statistical significance (P< 0.05), Smoking group T, FSH and LH content t-shirts were lower than the control group and eight weeks are statistically significant differences (P< 0.05); Stop smoking group T, FSH, LH content are below experiment smoking group before there were no statistically significant and differences(P> 0.05), Stop smoking group T, FSH, LH content were lower than that of the control group 12 weeks there were no statistically significant and differences (P> 0.05). Eight weeks prior to the control group and control group,12 weeks, FSH and LH content t-shirts were no obvious difference.2. Seminal plasma biochemical tests results:pure berries sugar, smoking set zinc content are relatively 8 weeks controls are down, differences statistical significance (P < 0.05). Pure berries sugar, smoking set zinc content than 12 weeks are pure berries group drop, sugar content and 12 weeks are statistically significant differences in the control group (P<0.05); Seminal plasma zinc content and 12 weeks was not statistically significant differences in the control group (P> 0.05).8 weeks of control group and control group 12 weeks of pure berries sugar, zinc content were not significantly different.3. Testicular HE dyeing results:eight weeks group with 12 weeks was testicular tissue pure epithelial polyimides complete, various born orderly sperm cells, administrative levels clear. Stop smoking group testicular pure epithelial polyimides born thinning, born at all levels than the control group reduce sperm cells, structure, hierarchy variable thin sparsely, tubular gap grows wider, mesenchymal cells decreases. Smoking group pure epithelial polyimides born at various levels broken dissolve, born disordered arrangement sperm cells, cells, the decrease in the number of smaller gap increases, cells, edema, mesenchymal cells further reduce.Conclusion:1. Cigarette smoke can lead to male rats serum follicle-stimulating hormone, testosterone, progesterone levels are low, tip:may interfere with hypothalamus-pituitary-adrenal axis function, testicular gonad born of the testes of pure role influence.2. Cigarette smoke can lead to male rats pure berries sugar, zinc content low, semen quality, likely to impact density, etc.3. This study is caused cigarette smoke causes of male infertility provide certain evidence diagnosis.