| Objective:To investigate Qiao-qin-qing-fei granule relieving asthma effect of the asthmatic rats induced by ovalbumin and its mechanism.Methods:Bronchial asthmatical rats were established by OVA-sensitized and repeated-inspiring method.91 rats were randomly divided into the control group, asthma group, dexamethasone group, aminophylline group and Qiao-qin-qing-fei (26g/kg,13g/k,6.5g/kg) group. Every rat except the control group was sensitized by injecting 10% of ovalbumin through intraperitoneal in the first day and the eighth day, while begin from the fifteenth day 1% of OVA aerosol inhalation(once a day, every time 20 minutes) for 14 days continuously, and the normal control rats were given the same amount of saline. In the second day of sensitization, the rats were administered with above-mentioned drugs by intragastric administration respectively, and the rats in normal group and model group were given the same volume of saline(once a day) for 4 weeks continuously. The degree and latency of asthma were tested in the process of inducing asthma; After 24h of the last nebulized inhalation, HE staining method was applied to observe the morphological changes of histologic lung tissues; Elisa was used to detect the contents of serum PAF and measur the levels of ET, IL-4, IL-5, IFN-r in bronchoalveolar lavage fluid; Detected total white blood cells and the content of eosinophils by blood drawing; Measured the expression of CD4+,CD25+ in peripheral blood was detected by flow cytometry.Results:(1) Asthmatic rats gasped significantly, shortened the latency of asthma; Compaed to control group, the content of serum PAF in model group had increased markedly (p<0.01), the level of ET, IL-4, IL-5 in bronchoalveolar lavage fluid had increased obviously (p<0.01,p<0.05) and the level of IFN-r had decreased significantly (p<0.01);Total white blood cells and the proportion of eosinophils in rat peripheral blood were higher than normal model(p<0.05); the presence of CD4+ in model group lower than normal group, but there was had no significant difference(p>0.05).(2) Compared to model group, the degree of asthma in high-dose qiao-qin-qing-fei group, middle-dose qiao-qin-qing-fei group, low-dose qiao-qin-qing-fei group, dexamethasone group and aminophylline group had decreased markedly and significantly extended the latency of asthma (p<0.01), while the levels of PAF had decreased in varying degrees (p<0.01).(3) Compared to model group, the content of ET had reduced greatly in dexamethasone group, aminophylline group and high-dose Qiao-qin-qing-fei group and middle-dose group (p<0.01), but there is had no significant effect in low-dose Qiao-qin-qing-fei group (p>0.05).(4) Compared to model group, the level of IL-4 had decreased obviously in high-dose Qiao-qin-qing-fei group, middle-dose Qiao-qin-qing-fei group, low-dose Qiao-qin-qing-fei group, dexamethasone group and aminophylline group at different degrees (p<0.01,p<0.05); the content of IL-5 had reduced only in dexamethasone group and high-dose Qiao-qin-qing-fei group (p<0.05); the level of IFN-r had increased significantly in all the treatment groups except low-dose Qiao-qin-qing-fei group (p<0.01).(5) Compared with model group, low-dose Qiao-qin-qing-fei group had no significant effect on eosinophils (p>0.05), but the other groups reduced total white blood cells and the proportion of eosinophils with different degrees(p<0.01,p<0.05).(6) Dexamethasone, aminophylline and different dosages of qiao-qin-qing-fei granule had no obviously effect on the expression of CD4+,CD25+.(7) Both of the bronchial epithelial cells and goblet cells hyperplasia of asthmatic rats were observed under microscopy, and there were secretion and inflammatory cells in pulmonary alveolar. After drugs treatment, bronchial epithelial cells and goblet cells hyperplasia had reduced. Furthermore, there were less secretions and cell infiltration within alveolar and the smooth muscle thickening was not obvious.Conclusions:1. Qiao-qin-qing-fei granule has the role of improving and delaying the onset of asthma induced by ovalbumin.2. The preventive and therapeutic effect of qiao-qin-qing-fei may be related with reducing total white blood cells and the proportion of eosinophils in peripheral blood, as well as the contents of serum PAF, decrease ET levels of lavage fluid, regulating the balancing of Thl/Th2 in asthmatic rats. |
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