The Study Of Establishment Of Abmormal Balgam Syndrome Impotence Modle Of Uygur Medicine

Objective:To study of establishment and material basis of abmormal balgam syndrome impotence modle of Uygur medicine. Methods:Selected SD rats of 100 mature male, Take out of ten for the control group,the other in the model.To established animal models of abnormal balgam syndrome by spinach and coriander method into the cold environment. Biological characterization of the rats, Experimental evaluation by APO erectile function experiment in rats, selected impotence model after 20 weeks.The impotence rats were randomly divided into modle group(A1), spontaneous recovery group(A2) and medication group(A3). The non-impotence rats were randomly divided into modle group(B1), spontaneous recovery group(B2) and medication group(B3). Continue to biological characterization of the rats, evaluate erectile function. After disproofed the modle for 2 weeks, the level of sex hormone of all groups were detected by radioimmunoassay. Expression of eNOS-mRNA,nNOS-mRNA in penis was detected by RT-PCR.Results:(1)At the period of model, the change in the model consistent with the general state of Uygur medicine general characteristics of abnormal balgam, that is lying curled up to less, like to get together, slow, dull hair, chaos, clear urine, pale stool the soft stool, dark tongue, greasy tongue coating, slow weight gain, increased food intake, decreased water intake, urine and stool output increased.(2)The first week of disproof, above-mentioned characteristics did not recover. The second week, A1 did not recover, the biological characterization of B1 is decreased food intake, increased water intake, urine and stool output decreased, lighten greasy tongue coating, the biological characterization of A3 and B3 is apparente decreased food intake, increased water intake, urine and stool output decreased, and red tongue. medication group recovered partial at the second week. (3) 1 weeks to 8 weeks of modeling, the erectile latency and the number of erections of model group has no different with normal group(P>0.05).8 weeks to 20 weeks of modeling, the erectile latency of model group had significant longer than in normal group(P>0.05), reduce the number of erections (P<0.05). Disproofed period, the erectile latency of A1,A2 are significant longer than in normal group(P<0.05), reduce the number of erections (P<0.05). The other model group have no different with normal group(P>0.05). The erectile latency and the number of erections between A1,A2 have no different(P>0.05), the erectile latency of A3 is shorter than A1, A2, the number of erections is more than A1, A2 (P<0.05).(4) The twentieth weeks of modeling, the incubation periods of insertion and ejaculation of model group is longer than normal group. And the frequency of straddle, insertion and ejaculation is lesser than normal group(P<0.05). Disproofed period, the incubation periods of insertion and ejaculation of A1,A2,B1 are longer than normal group. And the frequency is lesser than normal group(P<0.05). The incubation periods of ejaculation between A1,A2 has no different(P>0.05), the incubation periods of insertion and ejaculation of A3 are shorter than A1,A2, the frequency are lesser than them(P<0.05,P<0.05). (5) Testosterone:A1,A2,B1 are obviously lower than the normal group, the other model group have no different with normal group(P>0.05). A3 are higher than A1,A2(P<0.05), The level between A1,A2,B1 has no statistic significance(P>0.05).B2,B3 are higher than B1(P<0.05). LH:A1 is obviously higher than the normal group(P<0.05), the other model group have no obvious difference with normal group(P>0.05).FSH:each of group have no obvious difference(P>0.05). E2,PRL:A1 is obviously higher than the normal group and A3(P<0.05), other groups have no difference(P>0.05). (6)eNOSmRNA:The expression level of A1 is obviously lower than the normal group(P<0.05), the other model group have no obvious difference with normal group(P>0.05). The level between A1,A2 has no different(P>0.05), A3 is higher than A1, B3 is higher than B1(P<0.05,P<0.05). nNOSmRNA:The level of A1 and A3 is obviously lower than normal group(P<0.05), the other model group have no obvious difference with normal group(P>0.05). The level between A1,A2 has no different(P>0.05), A3 is higher than A1,A2, B3 is higher than B1(P<0.05,P<0.05). Conclusion:(1) The wet-cold environment and method of feeding intervention can be successfully cross-examined to establish abnormal balgam and abnormal balgam of climate models Syndrome impotence model, the change in the model consistent with the general state of Uygur medicine general characteristics of abnormal balgam. The erectile latency of model group is significant prolongation, mating ability decreased. The model mode is 56.7%. (2)Through spontaneous recover and Yimusake tablet treatment, the model is reliability and stability. (3)The level of Testosterone of abmonnal balgam syndrome impotence modle decrease significantly, and the level of LH,E2 and PRL is obviously increase. The functional disorder of gonad axis maybe concerned with abmormal balgam syndrome impotence modle of Uygur medicine. (4) Expression of eNOS,nNOSmRNA in penis of abmormal balgam syndrome impotence modle decrease significantly. The lower Expression of nNOSmRNA maybe the pathogenesis of abmormal balgam syndrome impotence.