BacT / ALERT 3D Culture System Of Mycobacterium, Rapid Detection Of Drug Resistance Genes In The Diagnosis And Treatment Of Spinal Tuberculosis

Objective BacT / ALERT 3D method of treatment of spinal tuberculosis in different periods, different lesions were cultured tissue specimens, polymerase chain reaction-single strand conformation polymorphism detection of positive samples of drug resistance genes of the two methods in the diagnosis and treatment of spinal tuberculosis in value. Analysis of Mycobacterium tuberculosis in the spine in the presence of hardened walls, from the etiological point of view for the surgical treatment of spinal tuberculosis to provide theoretical basis for improvement.Methods (1) conform to the requirements of this study 53 patients with spinal tuberculosis patients and 43 cases of surgical treatments in the collection of tuberculous granulation tissue, pus, and hardening of bone as a specimen, Were inoculated at three BacT / ALERT 3D liquid medium and three Roche culture medium, and the other 10 patients were admitted for a CT-guided abscess or vertebral puncture BacT / Alert 3D culture methods, after 2 weeks of anti-TB specimens were obtained from the same patients BacT / Alert 3D culture method. Positive luxuriant-Nepal, after acid-fast staining, PNB and TCH medium inoculated with strain identification. (2) BacT / ALERT 3D method PCR-SSCP detection of positive samples of isoniazid, streptomycin resistance gene katG and rpsl.Results (1) 43 patients with tuberculous granulation tissue, BacT / ALERT 3D culture method and the Roche culture positive rate 44% (19/43), 25% (11/43), pus BacT / ALERT 3D culture method and the Roche culture positive rate of 23% (10/43 ), 9% (4 / 43), BacT / ALERT 3D method of granulation tissue and pus-positive TB reported average time of 16 days for 19 days, Roche TB culture-positive granulation tissue and pus average reporting time of 30 days and 27 days, 19 cases were positive results Mycobacterium species identification of future generations of 18 cases of Mycobacterium bovis in 1 case. The other 10 patients received treatment of CT-guided abscess Or vertebral puncture BacT / Alert 3D method culture positive rate was 90% (9/10) (all human-type Mycobacterium tuberculosis), chemotherapy 2 weeks after surgery in the same specimen BacT / Alert 3D method culture positive rate was 20% (2 / 10) . (2) polymerase chain reaction-single strand conformation polymorphism in 11 clinical isolates of Mycobacterium tuberculosis, 2 cases of isoniazid-resistant strains, penicillin-resistant strain 4 Cases. The average reporting time 16 hours and 14 hours.Conclusion (1) BacT / ALERT 3D culture system of Mycobacterium tuberculosis is a rapid clinical pathogen isolated and cultured spinal tuberculosis better way, the positive rate with different treatment time, tumor tissue samples of different options are directly related. (2) Polymerase chain reaction - single strand conformation polymorphism 11 clinical isolates of Mycobacterium tuberculosis, isoniazid-resistant strains in 2 cases, 4 cases of penicillin- resistant strains. The average reporting time 16 hours and 14 hours. (3) hardening of the wall in the focus of spinal tuberculosis with tuberculous lesions persist after surgery can achieve a more complete lesion resection radical, while beneficial to bone block faster and better integration.