| ObjectiveBy observing the cardiac structure and function of the people who drinking heavy and less alcohol,while determination the content of reactive oxygen species(ROS) and the vigor of superoxide dismutase(SOD) and glutathione peroxidase (GSH-PX) in blood serum,to investigate the effect of drinking heavy and less alcohol to cardiac structure and function and the role of oxidative stress in alcohol cardiac muscle damage. Methods1.Study:From March 2009 to November 2009, the amount of people who participated in healthy check in North China Coal Medical College Hospital and Tangshan Iron and Steel Group Co.,Ltd.Hospital were about 2,000. Among those who meet the criteria for inclusion in this study of 162 cases. Inclusion criteria:(1) alcohol consumption: 5 ~ 20g / d, drinking time: 10 years and above;(2) alcohol consumption:≥80g / d, drinking time: 10 years and above. Exclusion criteria: coronary heart disease, diabetes, hypertension, liver cirrhosis patients, other known causes of heart failure patients, thyroid disease, chronic lung disease and smokers. Group(daily alcohol intake): (1) Less drinking groups: alcohol intake 5~20 g / d, 10 years or more consecutive drinks alcohol ,male, 66 c ases; (2) heavy drinking group:alcohol intake≥80g / d, 10 years or more consecutive drinks alcohol ,male, 56 cases;(3) Not Drinking Group: to participate in the examination of non-drinkers, non-drinking is defined as weekly consumption of alcohol content of less than 5 grams. Exclusion criteria with the alcohol group,male,40cases.2. Detecion: ELISA,Fenton and Griess color theory, hydroxylamine method,colorimetric were used to determinate BNP,ROS levels and SOD,GSH-PX vitality in blood serum of each group.3.Echocardiography: By experienced physicians to complete echocar- diography check,determination of indicators:intermediate septum thickness (IVS),Left ventricular end-systolic dime(LVSD),Left ventricular end-diastolic dime(LVDD),Left ventricular posterior wall systolic thickness(LVPWTs),Left ventricular posterior wall diastolic thickness(LVPWTd),Left ventricular ejection fraction(EF%),Left ventricular Fractional shortening(FS%),Mitral early diastolic maximum flow velocity(E peak),The maximum late diastolic flow velocity(A peak),and to calculate the ratio of E/A.4. Clinical indicators:All selected persons did associated history taking, comprehensive physical examination, relevant laboratory tests and X-ray, ECG.Results1.Comparison of normal subjects:There were no significant diff- erences between the heavy drinking group, less drinking group and the not drinking group in age ,sex, height, weight and blood pressure (p>0.05).2. Cardiac structural changes: The difference of IVS, LVPWTs, LVPWTd was not statistically significant among the three groups (P>0.05); Compared with not drinking group, the LVSD, LVDD of heavy drinking group increased,[(34.78±4.14)mm vs(27.85±3.58)mm(P<0.05) ],[(52.54±3.90)mm vs(45.92±2.81)mm(P<0.05)], the differences were significant.3. Cardiac function changes:(1)Compared with less drinking group, the EF, FS and E / A ratio of heavy drinking group decreased, respectively: [(61.15±3.69)% vs(69.23±4.15)%(P<0.05) ],[(30.62±3.31)% vs(36.15±3.68)%(P<0.05) ],[(1.14±0.15)vs(1.19±0.25) (P<0.05)] ,the differences were significant.(2)Compared with not drinking group, the EF, FS and E / A ratio of heavy drinking group decreased, respectively: [(61.15±3.69)% vs(68.08 method,colorimetric were used to determinate BNP,ROS levels and SOD,GSH-PX vitality in blood serum of each group.3.Echocardiography: By experienced physicians to complete echocar- diography check,determination of indicators:intermediate septum thickness (IVS),Left ventricular end-systolic dime(LVSD),Left ventricular end-diastolic dime(LVDD),Left ventricular posterior wall systolic thickness(LVPWTs),Left ventricular posterior wall diastolic thickness(LVPWTd),Left ventricular ejection fraction(EF%),Left ventricular Fractional shortening(FS%),Mitral early diastolic maximum flow velocity(E peak),The maximum late diastolic flow velocity(A peak),and to calculate the ratio of E/A.4. Clinical indicators:All selected persons did associated history taking, comprehensive physical examination, relevant laboratory tests and X-ray, ECG.Results1.Comparison of normal subjects:There were no significant diff- erences between the heavy drinking group, less drinking group and the not drinking group in age ,sex, height, weight and blood pressure (p>0.05).2. Cardiac structural changes: The difference of IVS, LVPWTs, LVPWTd was not statistically significant among the three groups (P>0.05); Compared with not drinking group, the LVSD, LVDD of heavy drinking group increased,[(34.78±4.14)mm vs(27.85±3.58)mm(P<0.05) ],[(52.54±3.90)mm vs(45.92±2.81)mm(P<0.05)], the differences were significant.3. Cardiac function changes:(1)Compared with less drinking group, the EF, FS and E / A ratio of heavy drinking group decreased, respectively: [(61.15±3.69)% vs(69.23±4.15)%(P<0.05) ],[(30.62±3.31)% vs(36.15±3.68)%(P<0.05) ],[(1.14±0.15)vs(1.19±0.25) (P<0.05)] ,the differences were significant.(2)Compared with not drinking group, the EF, FS and E / A ratio of heavy drinking group decreased, respectively: [(61.15±3.69)% vs(68.08 ±156.34)u/ml(P<0.05)],[(116.01±15.08)u/ml vs(75.46±33.94)u/ml(P<0.05) ],[(721.42±156.73)u/ml vs(437.23±45.84)u/ml(P<0.05)],the differences were significant.Conclusion1.The people who drinking heavy alcohol showed changes in cardiac structure and cardiac function decreased. The people who drinking less alcohol did not appear to cardiac structure and function changes.2.The serum levels of oxidative stress factor ROS increased and the SOD, GSH-PX activity reduced in the people who drinking heavy alcohol . The serum levels of oxidative stress factors ROS reduced and the SOD, GSH-PX activity increased in the people who drinking less alcohol.
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