Studies On Chemical Constituents Of Dicliptera Chinensis(L.) Nees And Active Extracts Of Polysaccharide On Liver Protection

The Dicliptera chinensis (L.) Nees( Acanthaceae ) regionally distributes in Guangxi, Guangdong, Fujian, Anhui as one of the common wild herbs in South China. The dicliptera chinensis can clear heat and detoxicate, cool the blood, promote saliva or body fluids, improve the diuresis function, used in the fever macula, acute hepatitis, having blood in stool, drowning the blood, the difficult urination, swelling and furunculosis and so on.. Up to now, there was little report about the chemical constituents and bioactivities of Dicliptera chinensis (L.) Nees.In order to elucidate the constituents of this plant, fourteen compounds were isolated from the 75% EtOH extract of Dicliptera chinensis by means of the repeated column chromatography on silica gel, recrystallizing. Their chemical structures were elucidated on the basis of physico-chemical properties and spectroscopic analysis, including IR,UV,TLC,TOF-MS, 1H-NMR, 13C-NMR etc. These compounds were(1)1-octadecanol, (2 )Epicatechin(,4)Diosgenin, (5)Chlorogenic acid, (6)Ferulic acid, (7 )Gardenoside , (8)Ursolic acid, (9)6-hydroxycoumarin, (10)Isoalantolactone, (11)Oleanic acid, (12)Daphnoretin, (13)Scoparone, (14)Daucosterol, (15)β-Stitosterol, (16)Kaempferitrin。Among them, compound 2,4,5,6,7,8,9,10,13,16 were isolated from this genus for the first time.We also Compared the function of different molecular weight polysaccharide of Dicliptera chinensis to decrease the enzyme action. Uses the carbon-tetrachloride (CCl₄)- induced rats acute liver damage model, observe the content of dicliptera chinensis different molecular weight polysaccharide to the Alanine aminotransferase (ALT) activity, aspartate aminotransferase (AST) activity, the total protein (TP) content in blood serum; change in liver superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activeness, malondialdehyde (MDA) content and liver morphological changes; effect on organindexThe results shows:(1)Various polysaccharides-treated group with CCl₄-treated group and find significant difference in rats's serum AST and ALT activities and group with the molecular weight <6000 has the most remarkable effect (P<0.01)which was comparable to that of the reference drug, bifendate.(2)The group with molecular weight <6000 polysaccharide and the total polysaccharide group may obviously reduce the rise of TP(P<0.05), and the group with molecular weight <6000 has superior effect compared with the total polysaccharide group(P<0.05) and the positive medicine group(P<0.05).(3)The group with molecular weight <6000 can enhance the decreased activity of SOD in liver(P<0.01).(4)Total polysaccharide group and group with molecular weight between 6000 and 10000 polysaccharide can remarkably enhance the decreased activity of GSH-Px in liver(P<0.01), and the effect was comparable to that of the reference drug, bifendate.(5)The MDA content in rats liver in various polysaccharides group remarkably decreases compared with that of the model control group(P<0.01), among which the total polysaccharide group, the molecular weight <6000 group and the molecular weight >10000 group have superior effect to that of bifendate group, and the effect of the total polysaccharide group is most superior(P<0.01).(6)According to the microscopy, the dicliptera chinensis polysaccharide may reduce CCl₄-induced acute liver damage. The dicliptera chinensis total polysaccharide and 3-section molecular weight polysaccharides have remarkable effect on rats acuite liver injury caused by the carbon tetrachloride.(7)In all, The group with the molecular weight <6000 has the most remarkable effect which was comparable to that of the reference drug.In conclusion, this paper provided valuable experimental data for further development of Dicliptera chinensis.