Effect Of Lactobacillus Plantarum In Expression Of Tight-junctional Molecules In Rat Hepatocytes During Common Bile Duct Ligation

Objective To investigate the effects of Oral Lactobacillus Plantarum supplementation on Liver function, endotoxemia, apoptosis of liver and tight junction of hepatocytes in an experimental model of obstructive jaundice.Method A total of 50 male Wistar rats were randomly divided into five groups of 10 each :â… ,sham-operated;â…¡,bile duct ligation (BDL);â…¢,bile duct ligation +LP (0.2g/5ml/day);â…£, bile internal drainage (IBD);â…¤,bile internal drainage +LP. Hepatocyte apoptosis was quantitated using the transferase-mediated uridine nick end labeling (TUNEL) assay. Portal blood samples for endotoxin measurements were obtained 10 days later. Transmission electron microscopy, RT-PCR and Western blot to observe the ultrastructure and distribution of tight junction associated proteins Claudin-1, Occludin, Claudin-4, ZO-1.Results Compared to the groupâ… ,a significant increase in Hepatic enzyme, endotoxemia, apoptosis (P<0.01). After oral administration of LP the situation alleviated. Bile internal drainage did great help of liver but compared with groupâ…¤the lat has the more effect. The TJ have been evidently destroyed under electron microscope, after oral LP the junction improved. The TJ of groupâ…£is better then the groupâ…¡but the groupâ…¤has the best amendment. The consequence of RT-PCR and Western blot has the same change.Conclusions Oral Lactobacillus Plantarum in Rats with Obstructive Jaundice assist the liver to resume, active suppression hepatic Barrier function injury; supply a path for the prevention in rats with obstructive jaundice...