Studies Of The Effect Of The Signal Pathway Of NF-κB On Airway Inflammation In Mice Asthmatic Model And The Modulating Effect Of The Traditional Chinese Medicine For Asthma

Studies of the effect of the signal pathway of NF-κB on airway inflammation in mice asthmatic model and the modulating effect of the traditional Chinese medicine for asthmaObjective To explore the effect of the signal pathway of NF-κB on airway inflammation in mice asthmatic model and the modulating effect of the traditional Chinese medicine for asthmaMethods 60 healthy female Kunming mice were divided into six groups randomly,10 mice of each group, normal control group (A), asthma group (B), pulmicort group(C), Ginseng-Schisandrae decoction group (D), Xiao qinglong Decoction group (E), two step sequential therapy group(F). The mice of B,C,D,E,F groups were established the models of asthma using ovalbumin, after building, each group were given differently therapy. Then the changes of pathological in lung tissue and bronchoalveolar lavage fluid (BALF) in cytology were observed.Immunohistochemical analysis was used to detect the level of GATA-3 and nuclear factorκB(NF-κB) in lung tissue.Enzyme-linked immunosorbent assay(ELISA) was used to assay the levels of IL-4 in BALF.Results①There was no wheezing symptoms in group A while there were wheezing in other groups and group B were more serious than the treatment groups.There was no significant different between the treatment groups.②Pathological analysis of the lung showed that A group had a little inflammatory cell infiltration and was significantly lower than other groups(P<0.01) while B group had a large number of inflammatory cells infiltrating around tracheas and was significantly higher than other groups(P<0.01).There was no significantly difference between the treatment groups(P>0.05).③Number of total leukocytes and the proportion of eosinophil, neutrophil, lymphocyte of group A in BALF were significantly lower than other groups respectively (P<0.01) while that of group B were significantly higher than other groups respectively (P<0.01). There was no significant difference between treatment groups(P>0.05). The proportion of phagocyte of group A in BALF was significantly higher than other groups (P<0.01) while that of group B was significantly lower than other groups (P<0.01). There was no significant difference between treatment groups(P>0.05).④The NF-κB of lung tissue were lower in group A than that of other groups (P<0.01) while that of group B were higher than that of other groups (P<0.01). There was no significant differences between the treatment groups(P>0.05).⑤The GATA-3 of lung tissues were lower in group A than that of other groups(P<0.05)while that of group B were higher than that of other groups (P<0.01). There was no significant differences between the treatment groups(P>0.05).⑥The levels of IL-4 in BALF were significantly lower in group A than that of other groups (P<0.01) while that of group B were higher than other groups (P<0.01) and there was no significant differences between treatment groups(P>0.05).Conclusions The model of asthmatic mouse stimulated by OVA may be resulted from NF-κB of lung tissue expressing excessively, which result signaling pathways activated and the GATA-3 expressing excessively. Then Th2 is produced excessively, which result inflammation around the tracheas.Traditional Chinese medicine and glucocorticoid may reduce airway inflammation by lowering the expression of NF-κB, which inhibit the active of signal pathway and the expression of the GATA-3.