Study On Extraction, Isolation And The Molecular Structure Tracing Of Active Components From Several Medicinal Plants

This study was based on a key project of Shandong Provincial Natural Science Foundation which had been completed by Shandong Academy of Agricultural Sciences Central Laboratory. A variety of medical resources with development and utilization value, which contained a great deal of highly active substances were selected out, including Aristolochia mollissima Hance, Raphanus sativus L., Rehmannia glutinosa, Houttuynia cordata Thumb. The objective was to get a novel bioactive compound or a lead compound, in order to provide theoretical basis for new drug discovery, research foundation for development and utilization of these medical plants.On the base of searching and analyzing the interrelated data related to the chemical constituents and pharmacological study of Aristolochia mollissima Hance, Raphanus L., Rehmannia glutinosa, Houttuynia cordata Thumb. home and abroad, the author was studying on the fingerprints of Aristolochia mollissima Hance, Raphanus sativus L., Rehmannia glutinosa, Houttuynia cordata Thumb., the determination of amino acid contents in Aristolochia mollissima Hance, Raphanus sativus L., Rehmannia glutinosa, Houttuynia cordata Thumb.,the chemical constituents of Aristolochia mollissima Hance., the chemical constituents of Raphanus sativus L. The results are as follows:1. Study on the fingerprints of Aristolochia mollissima Hance., Raphanus sativus L., Rehmannia glutinosa, Houttuynia cordata Thumb by hplc. In 60 minutes, all the extracts from each sample were well separated on C18 column, with a good reproducibility. A more feasible and reliable experimental method was established. Chromatography was set up with gradient elution and low-wavelength detection. There were 41 common peaks in the fingerprint of Aristolochia mollissima Hance,the similar degree above 0.9. 8 common peaks in the fingerprint of Raphanus sativus L. 7 common peaks in the fingerprint of Rehmannia glutinosa. 12 common peaks in the fingerprint of Houttuynia cordata Thumb. The fingerprint of Raphanus sativus L. hasn't been reported so far.2. The amino acid contents in Aristolochia mollissima Hance, Raphanus sativus L., Rehmannia glutinosa, Houttuynia cordata Thumb were determined by Biochrom 30. The results showed that: there were large amounts of amino acids in all plants, among which 17 kinds were detected under the experimental conditions. The total amino acid content in Aristolochia mollissima Hance, Raphanus sativus L., Rehmannia glutinosa and Houttuynia cordata Thumb were 6.43587 g/100 g, 17.71729 g/100 g, 3.15016 g/100 g, 6.90345 g/100 g respectively. The amino acid contents in green radish were 3-5 times higher than the others. The contents in Aristolochia mollissima Hance was similar to in Houttuynia cordata Thumb.3. The dried Aristolochia mollissima Hance was extracted with industrial methanol at room temperature, the combined extracts were vacuo and then partitioned with petroleum ether, ethyl acetate, n-butanol, the part of chloroform extracted was isolated by silicagel colum chromatography, Sephadex LH-20 colum chromatography, recrystal etc. to obtain 1 compound from the petroleum ether part and 4 compounds from the ethyl acetate part, the structures of which were elucidated by the physicochemical properties and spectroscopic analysis (1H-NMR,13C-NMR) as Aristololactone(I), Aristolochic acid A(II), Aristoloterpenate I,β-sitosterol, Versicolactone B.4. The concentrated industrial methanol extractor from the Raphanus sativus L. was suspended in H2O and partitioned with petroleum ether, ethyl acetate, n-butanol for the first time. The chemical constituents of Raphanus sativus L. were isolated and purified by solvent extraction and column chromatography. As a result, 3 compounds from the petroleum ether part were isolated and identified by using physicochemical properties, spectral analysis (1H-NMR,13C-NMR), identified as:β-sitosterol, linum cerebroside, obtained from this genus for the first time.β-sitosterol was isolated from the ethyl acetate part either.