Money Grass Chemical Composition And Quality Standard

Lysimachia christinae Hance. is known as a traditional ChineseMedicine for treating stranguria and calculus. And its main localityis Sichuan Province.In this experiment, we made microscopic and macroscopicidentifcation for Lysimachia christinae Hance., Desmodiumstyracifolium(Osb.)Merr., Glechoma longituba (Nakai)Kupi.,Lysimachia hemsleyana Maxim., and Lysimachia congestifloa Hemsl..Lysimachia christinae Hance., Desmodium styracifolium (Osb.)Merr.and Glechoma longituba (Nakai) Kupi. are the varieties which areincluded by Chinese Pharmacopeia, while the last two varieties areconsidered as the fake.Using choleresis as a indicator, to find active components ofHerba Lysimachiae. Researched water extract and ethanol extractfrom Herba Lysimachiae on choleresis of rats. The results indicatedethanol extract from Herba Lysimachiae was able to stimulate thesecretion of bile of rats, gallbladder smooth muscle relaxation, andenhance the gallbladder emptying.From the alcohol extract of Lysimachia christinae Hance., fivecompounds were isolated by various chromatographic methods. On thebasis of analysis of UV, NMR, MS data and measuring ofphysico-chemical characters, the structures of five compounds wereidentified as kaempferol, quercetin, stigmasterol,kaempferol-3-0-glucoside, quercetin-3-0-glucoside.Using rutin as reference substance a UV spectrophotomatry wasdeveloped to determine total flavonoid in Lysimachia christinaeHance.. Setting up the quality control standard of Lysimachiachristinae Hance..Using kaemperfol as index ingredient first and through HPLC, weset up the fingerprinting of Lysimachia christinae Hance..