| Objective: Different kinds of articular cartilage injury and articular cartilage diseases may lead to articular cartilage defects. As well as there is not direct vessels, lymphatic vessels ,nerves in articular cartilage and the physiological nature of low metabolic rate,it is difficult rehabilit itself .So,up to now ,it is a question which medical circles home and abroad still pay attention to and need badly resolutions. Now, all the therapmatic methods of healing of full-thickness defects of articular cartilage are in reseach. There are many reports about how to improve the quality of healing of full-thickness defects of articular cartilage,however, there are shortcomings in every therapmatic method. In this experiment, we make the modols of the man-made full-thickness defects of articular cartilage in rabites through drilling into the bone below articular cartilage to observe the process of repairing full-thicknessd defects of articular cartilage and study effect of regular passive motion(RPM) on the healing of man-made full-thickness defects of articular cartilage in rabit knees.Methods: 3.2mm diameter full-thicknessd defects of articular cartilage in the facies patellaris of femur were made in 30 male New Zealand white rabbit knees. Then the rabbits were randomly divided into three groups, A group:8h/day RPM(onece every 20 minutes, and last for 2 minutes ,60times/minute,4hours in the morning and 4hours in the afternong everyday) and normal cage activity; B group:2h/day RPM (onece every 20 minutes, and last for 2 minutes, 60times/minute, 1hour in the morning and 1hour in the afternong everyday) and normal cage activity; and C Group: normal cage activity only. There were ten rabbits and twenty knees of male New Zealand white rabbits in each group, half of each group were sacrificed at the end of four and twelve weeks.Weight them respectively and compare with eachother among three groups.To observe gross appearance,the sections of full-thickness defects were stained by Hematoxyli-Eosin (HE) staining to observe histological structure, safranin-O staining to investigate expression of the glycosaminoglycan and immunohistochemical method to investigate the expression of collagen II, to score histological and histochemical result.Results: 1 There was no significant diference in the mean weight and the mean increased weight among three groups at preoperative period, RPM for 4 weeks and RPM for 12 weeks (P>0.05). 2 The result of Hematoxyli-Eosin(HE) staining in articular cartilage of each group comparing with normal articular cartilage, The specimens in group A are consistent of chondroid tissue,the apperences of which are temper rolling .The morphouses of cells are similar with normal chondrocytes. Cell-layers are obviovs, The collocation of cells is regular. The inferior tidal line of articular cartilage recovers on the whole. The specimens in group B are maily consistent of chondroid tissue, the appearances of cartilage are basically temper rolling . Cells in recovery tissue are fewer than the cells of articular cartilage. The collocation of cells is not so regular. Cell-layers are not so obviovs. The inferior tidal line of articular cartilage partly recovers. In group C, we can see much of fibrous tissue recovery. The appearances of cartilage are not flat.The two extremitats of defect meromixis with normal articular cartilage. The cells morphouses are obviously different to normal Chondrocytes. The collocations of cells are not regular. Cell-layers are not obviovs. The inferior tidal line disappead.There was significant diference between scores of gross appearance and histological change in A group and those in B group and the C group (p<0.05) but no significant diference between the Group B and the Group C (P>0.05). 3 Comparing the result of safranin-O staining of each group among three groups when RPM for 4 weeks and RPM for 12 weeks respectively, Then Comparing safranin-O staining of each group with each other.α=0.05,the result as follows: the safranin-O staining of group A is deeper than that of Group B and the Group C . There was no significant diference in Group B and the Group C.4 The immunohistochemical results are as follows: RPM for 4 weeks ,there are 12 specimens altogether of collagen II expression in the cartilage defects, of the total, groupA: 6 specimens, groupB: 4 specimens, groupC: 2 specimens. postRPM 12 weeks, there are 16 specimens altogether of collagen II expression in the cartilage defects, of the total, groupA: 9 specimens, groupB: 5 specimens, groupC: 2 specimens . There was no significant diference among three Groups in expression of collagen II,when RPM 4 weeks. However,RPM for 12 weeks, expression of collagen II in group A was higher than that of group B and group C ( p<0.05), however, there was no significant diference between group B and group C(p>0.05).Conclusions: 1. The healing of full-thicknessd defects of articular cartilage in rabbit knees treated by RPM is beter than that in rabbit knees without RPM treatment. There is significant diference between the healing efficiency in rabbit knees treated by RPM and that in rabbit knees without RPM treatment. RPM can stimulate healing of collagen II and the glycosaminoglycan. 2. RPM can stimulate the healing of full-thickness defects of articular cartilage in rabbit knees . The healing efect is relative to the period of RPM treatment.The efect of 8h/day RPM treatment is beter than that of 2h/day RPM treatment . |
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