A Variety Of Immune Analysis Methods For Quantitative Detection Of P53

Many scientific research groups have carried out extensive study on the structure of the immune system. imunoassay has been used diffrenent quantitative analysis to test analyte with labels to amplify signal, like nanomaterial used widly, which can amplify the signal in biological detection owe to their various properties. Simple and quick method in medical diagnosis, food analysis, medical inspection, and the application of envioromental protection and the other fields has great prospect. In accordance with the current trend of social development and needs, to create a novel, portable, eddicient, low-cost method is extremely important, so we used different methods to quantitative detection of phosphating protein p53.In our life p53 is associated with a lot of tumor and cancer. We tried to use the following three methods to realize the detection and devoted to exploring better use and real life, the experiment methods are as follows:1. Our experiment was conducted on the test strip covered the test line by Fe3O4-p5315 complex, under which we use magnetic to immobilize the Fe3O4-p5315 complex. Then, we drop the p5315 antigen on sample pad, and the antigen follow on the trip by the capillary action. Just moments later, test line could exist the self-assemble product which we cut off, and disposed by Triton X-100 to pyrolysis liposome, which finally detected by PGM. This method highlighted the ITS and PGM of the portable and convenient operation consumption.2. Our experiment used ZnO QDs as the electrical signal source. ZnO QDs was considered as a "green" material because of its large surface area, good biocompatibility and nontoxicity, and the weight ratio of loaded drug to carriers of GO mostly reach 200%, GO can load a lot of ZnO QDs. So chosing GO-ZnO QDs as signal amplification and magnetic nanoparticales as a base, then we detect electrical signal by SWV and obtain a lower detection limit. This method were low cost, easy preparation and the experiment using magnetic beads(MBs) facilitated the washing operation, and process efficiently.3. This experiment method was to explore another properties of ZnO QDs, unlike experiment 2, ZnO QDs were fluorescenct, in order to amplify the signal, so we choose two kinds of fluorescent particles in combination with each other for research to observe the fluorescence of superposition type reinforced. GQDs and ZnO QDs form QDs cluster, and the preparation of each other were simple, the use of reagents and equipment were very common, it based the foundation of the experiment which we developed smoothly, and fluorescence detection, without complex data processing, was widely application of detection period features.Experiment 1 focuseed on detection of convenience and portability to the development of practical application analysis, experiment 2 and 3 were for ZnO QDs application of this relatively new material to two aspects, on the one hand was its electrochemical properties which could be used to detect low contents of material, on the other hand was using the fluorescence characteristics to quickly detect and without complex post-processing. Whether people used was a method that need to be affordable, less pollution to the environment to the development of long lasting.