Shamisen, Traditional Chinese Medicine, Anti-tumor Activity And The Active Ingredient

It is widely recognized by the whole world that traditional Chinese medicine are useful to cure cancer. Some traditional Chinese medicine, like Dioscorea bulbifera L, Hedyotis diffusa Willd and Scutellrla barbata D.Don, have been proved to be of high curative effect during a long time. This thesis focused on the effective chemical components of these three medicines, separation and purification of Dioscorea bulbifera L and two traditional Chinese medicine compounds under the different condition. It was determinated the contents of polysaccharide and flavone, used anti-tumor cells activity test of every segment outside the body to filter the effective components, analysed the chemical compounds of active segments.Dioscorea bulbifera L was extracted by supercritical fluid extraction CO₂ (SFE-CO₂). The extraction have been test the activity of the anti-tumor cells outside the body. The conclusion is proved that the non-polarities of Dioscorea bulbifera L have high curative effects on anti-tumor. Then GC-MS was used to analyze the extractions. It was found that the extraction contain 33 kinds of compound accounting for 37.71%, including terpenoid, aliphatic compound, aromatic compound. Six terpenoid were isolated and identified by GC-MS, which are (R) caryophllene; (R) 4a,7-methano-4aH-naphth[1,8a-b]oxirene,octahydro;(R)azulene, 1,2,3,4,5,6,7,8-octah ydro-1,4-dimethyl-7-(1-methylethenyl);(M)-spathulenol;(R)naphthalene,1,2,3,4,4a,5, 6,8a-octahydro-7-methyl-isoprophl;(R)naphthalene,1,2,3,5,6,8a-hexahydro-4,7-dimet hyl-1-isoprophl.Dioscorea bulbifera L and two traditional Chinese medicine compounds were determined the contents of polysaccharide and flavone. Dioscorea bulbifera L and Chinese Angelica compounds' polysaccharide content was 16.32% by H₂SO₄-phenol method, the highest one. Three kinds of polysaccharide have certain anti-tumor activity by test. The order of anti-tumor activity was that Dioscorea bulbifera L and Chinese Angelica compounds first, Dioscorea bulbifera L second, Hedyotis diffusa Willd and Scutellrla barbata D.Don compounds third. The flavone content of...