Simple Sugars And Cellobiose High Performance Liquid Chromatography (hplc) Chiral Stationary Phase

The main works of the thesis were listed as follows:A introduction for chiral separation was given.Bonding glucose and cellobiose to silica gel via 3-aminopropyltriethoxysilane, 3-glycidoxypropyltrimethoxysilane or 3-isocyanatepropyltriethoxysilane, five chiral columns were prepared for HPLC. With the eluent of n-hexane/isopropanol=90:10, n-hexane/isopropanol/triethylamine=90:10:0.2(v/v) or n-hexane/isopropanol/trifluor-acetic acid=90:10:0.2(v/v), glucose chiral column appeared the best enantioselectivity when 3-aminopropyltriethoxysilane was used linkage arm.Arabinose, ribose, ribodesose, lyxose and fucose were first used as chiral selector which bonded to silica gel via 3-(triethoxysilyl)propyl isocyanate in HPLC. Some racemates were separated on these chiral stationary phases using hexane/isopropanol (90:10, v/v) as mobile phase. Those chiral stationary phases possessed good enantioseparation selectivity in the normal-phase mode, and there was a big chiral discriminating complementary. This work indicates that monosaccharide could soon become very attractive as a new kind of chiral stationary phase for HPLC.Four new chiral columns from monosaccharide derivatives were prepared. Some racemates were separated on these chiral stationary phases using hexane/isopropanol (90:10, v/v) as mobile phase by HPLC. However, these chiral stationary phases possessed low enantioselectivity.