The Expression Of The Angiopoietin And Vascular Endothelial Growth Factor (vegf) In Cervical Cancer And The Relationship With The Neogenetic Blood Vessel Density And Lymphatic Density

Objective: To explore the relationship between the expression of angiopoietin1,2 (Ang-1, 2) and vascular endothelial growth factor (VEGF) in cervical squamous cell carcinoma (SCC) tissue and microvessel density (MVD) as well as micro-limphatic vessel density (LVD ). To assess the role of the coexpression of Ang-1, 2 and vascular endothelial growth factor (VEGF) in the SCC played in tumor angiogenesis and the generation of lymphatic vessels of the tumor. in order to provide a new cervical cancer prevention and treatment approach.Methods: Immunohistochemistry SP method was used to detect the expression of Ang-1, 2 and vascular endothelial growth factor (VEGF) in 15 cases of normal cervical epithelium (NCE), 20 cases of cervical intraepithelial neoplasia (CIN), 49 cases of cervical squamous cell carcinoma with simple treatment of operation (SCC-O), as well as 43 cases of cervical squamous cell carcinoma treated with preoperative radiotherapy,chemotherapy and followed operation (SCC-RCO) , in the purpose of investigating their relationship with microvessel density (MVD) which was marked with CD34 and micro-limphatic vessel density (LVD) which was marked with D2-40.Results:1. From the NCE to the CIN to SCC-O group, the MVD value labeled with CD-34 was significantly higher with progress of the occurrence of cervical cancer (P <0.05). Preoperative treatment can significantly reduce the MVD value,of local tumor and had no significant correlation. With selection of radiotherapy or chemotherapy,Local LVD value with cervical cancer incidence was significantly higher with progress of the occurrence of cervical cancer (P <0.05),preoperative treatment can significantly reduce the LVD value of local tumorand had nosignificant correlation selection of radiotherapy or chemotherapy,.2. expression of Ang-2 and VEGF was in the law of similarity,.The positive expression rate of Ang-2 and VEGF significantly increased from the NCE to the CIN to SCC-O group (P <0.05), but there were no differences in the stageⅠand stageⅡSCC-O expression rates. Preoperative therapy can significantly reduce the expression of Ang-2 and VEGF protein (P <0.05), but preoperative radiotherapy or chemotherapy was no significant difference to reduce its expression. The expression rates of the Ang-2 and VEGF protein were associated with the lymph node metastasis of the cervical squamous cell carcinoma and the invasion depth of the stromal (P <0.05).3 Ang-1 protein mainly located in the cytoplasm of tumor cells, with low expression in the NCE group,but in CINⅢ, cervical squamous cell carcinoma of stageⅠ,Ⅱcases ,the level of positive expression was significantly higher than the NCE group (P <0.05); However,there were no significant differences in the positive expression rates in the three groups (P> 0.05);the positive expression rates in the SCC-O, SCC-CO and the SCC-RO group, were not obviously different either. The expression rate of Ang-1 protein was not determined by these pathological parameters such as the age of the patients with cervical squamous cell carcinoma, FIGO stage, lymph node metastasis and invasion depth of the stromal (P> 0.05). In the invasive cervical cancer, preoperative radiotherapy and chemotherapy on Ang-1 expression had no significant effects.4. MVD and LVD in cervical cancer has some relevance,It was significantly positively correlated,between Ang-2 and VEGF expression intensity and MVD value and the value of LVD .Ang-1 / Ang-2 and MVD value and LVD values was significantly negative correlation; To multi - stepwise regression analysis shows that the role of Ang2 on the MVD may be greater than VEGF, and VEGF on the role of LVD may be greater than Ang-2. Conclusion: High expression of Ang-1, Ang-2 and VEGF in cervical cancer might play an important role in the in tumor advancement. Mutual synergism of Ang-2 and VEGF had intimate relationship with the generation of cervical blood vessels and lymphatic. Cervical cancer radiotherapy and chemotherapy could significantly inhibit the formation of blood vessels and lymphatic vessels intumor tissue.