The Study On Relationship Between Genetic Polymorphisms Of O~6-methylguanine-dna Methyltransferase Gene And Esophageal Cancer In Kazakh In Xinjiang

Objective To explore relationship between genetic polymorphisms of O~6-Methylguanine-DNA methyltransferase (MGMT) gene and esophageal cancer in Kazakh in Xinjiang. Methods Codon 84C>T(L84F),Codon 53C>T(L53L),Codon 143A>G(I143V),Promoter 135G>T,Promoter 485C>A of MGMT gene in 160 individuals including 51 esophageal squamous cell cancer (ESCC) patients and 109 healthy controls of Kazakh in Xinjiang was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) based on case-control study strategy. Results The proportion of genotypes for the five single nucleotide polymorphisms (SNPs) of MGMT gene in ESCC group was similar to the controls group (χ2and P were 0.128, 0.938; 2.120, 0.346; 1.773, 0.183; 2.440, 0.295; 3.961, 0.138, respectively). Heterozygote and mutation genotype compared with wild genotype, the proportion for Promoter 485C>A of MGMT gene in ESCC group was different from controls group (χ~2=3.845, P=0.050). Calculate by logistic regression analysis (Adjusted age and sex), the proportion for the alleles of Promoter 485C>A in ESCC group was different from controls group also (P=0.018). Combine the five SNPs, the frequencies of 0 allele variant and 1-10 allele variants were 21.6% (11/51), 78.4% (40/51) in cases and were 37.6%(41/109), 62.4%(68/109) in controls, respectively. The proportion in ESCC group was different from controls group (χ~2=4.078, P<0.05), logistic regression analysis showed that 1-10 allele variants was an increased risk for the occurrence of ESCC, compared with 0 allele variant (adjust OR=2.632; 95%CI:1.127-6.105). Stratification analysis between the combine genotypes of the five SNPs and risk of ESCC, in young subjects (≦60 years), the proportion of 0 allele variant and 1-10 allele variants in ESCC group was different from controls group (χ~2=5.756, P=0.016), logistic regression analysis showed that 1-10 allele variants was an increased risk for the occurrence of ESCC, compared with 0 allele variant (OR=3.411; 95%CI: 1.204-9.663). Conclusions Promoter 485C>A of MGMT gene was associated with ESCC, but the other four SNPs may be not associated with ESCC. The five SNPs of MGMT gene may jointly poly a role in the susceptibility of ESCC, allele variants increased risk of the occurrence of ESCC in Kazakh in Xinjiang, and especially in young people (≦60 years).