Effects Of Qiliqiangxin On The Cardiac Function And The Expression Of Caspaes-3 In A Congestive Heart Failure Rat Model

Purpose: Evaluation of Qiliqiangxin capsule for improving cardiac function And Identification of Qiliqiangxin capsule for direct cytoprotective effects against Apoptosis for cardiomyocytes.Methods: Sprague Dawley (SD) rats were tested as subjects and the model rats were built by anterior descending coronary deligation, then the survival rats were devided into 6 groups randomly. sham-operated group(sham,n=8):fed with normal saline of same volume instead ;control group(control,n=7):fed with normal saline of same volume instead;Qiliqiangxin great dosage group(great,n=9):Qiliqiangxin 1.2g/kg d treatment;Qiliqiangxin medium dosage group(medium,n=9):Qiliqiangxin 0.6g/kg d treatment;Qiliqiangxin small dosage group(small,n=8 rats):Qiliqiangxin 0.3g/kg d treatment;the captopril group(captopril,n=9):captopril 25mg/kg d treatment.24 hours later, sham operated group and model group were given normal saline,treating group were given Qiliqiangxin, the contrasting group was given captopril.After treated 4 weeks, all rats'heart function were measruated by right flank arteria carotis communis arterial cannula, the left ventricle mass index was calculated and the expression of protein caspase-3 was tested by immunohistochemical method and Westernblot to assess the effect of qiliqiangxin.Results:1. Qiliqiangxin capsule could reduce effectively BW,HMI,LVMI compared to those of the control group.2. Declined LVEDP and LVSP,+dp/dtmax and -dp/dtmax of The Qiliqiangxin treatment groups increased significantly compared to those of the control group(P<0.05),The great dosage group increased significantly compared to the medium dosage Group and the small dosage Group (P <0.05).3. Immunohistochemical analysis and Westernblot analysis showed that adminstration of Qiliqiangxin significantly inhibited caspase-3 expressions in myocardial cells in a Conclusion: Qiliqiangxin significantly improved the CHF rat's cardiac function and reduced the left ventricle mass index and heart mass index 4 weeks after treated with relative high dose Qiliqiangxin. It might be partially attributed to the suppression of caspase-3 expressions.