Speed ​​hypoglycemic Experimental Diabetic Rats Early Peripheral Neuropathy Preventive And Therapeutic Effects

ObjectiveWe established the rats in diabetic peripheral neuropathy model to observe the effects of the Su-Jiang-Tang(SJT) on the blood glucose, body weight, blood TG and T.chol, hemorrheology, expression of NGF, the nerve conduction velocity and ultrastructure in these rats. This experiment was to investigate the mechanism of the protective effect on the early diabetic peripheral neuropathy of SJT and provide an experimental basis for further investigating and clinical use.MethodThe diabetes rats were induced by injecting Streptozotocin(STZ) into their abdomen successfully, and they were stochastic divided into five groups: diabetic model group "model group" (12 rats),low dosage of SJZ group "low dosage group" (10 rats),high dosage of SJT group "high dose group "(10 rats),Metformin group "drug I group"(10 rats), Metformin and Losartan group "drugn group"(10 rats); the other 10 normal rats were the normal control group "normal group". The respective dosage of drugs was given to the treated groups and the same dose of sodium chloride injection was given to the normal and model series by stomach rearing once per day for eight weeks. After therapy, the blood glucose, body weight, blood TG and T.chol,hemorrheology.expression of NGF.the nerve conduction velocity and ultrastructure of each group were tested.Result1 Body weight There were no significant differences among all group before treatment(P> 0.05).After treatments, the body weight of the high dosage group was distinctly heavier than that of the model group, drug I group , drugn group , respectively(P<0.01or P<0.05) .2 Blood glucose There were no significant differences among the blood glucose of all the treatment and model groups. The blood glucose of every treatment group was more lower than that of the model group(P< 0.05) .The blood glucose of the high dosage group was more lower than that of the drugI group ,drugn group , respectively(P<0.01 ) .3 TG and T.CH The TG and T.CH of every treatment group was more lower than that of the model group(P<0.05) There were no obvious difference among the low dosage group, the high dosage, the drug I and drugn group.4 Hemorrheology The whole blood viscosity, index of red cell(RBC) aggregation and index of RBC rigidity of model group was more higher thanthat of the normal group. The hemorrheological parameters of the high dosage and the drug n group was significantly decreased .There was no obvious difference among the high dosage group and drugll group.5 The nerve conduction velocity The nerve conduction velocity (NCV) of Every treatment group and model group was more slower than that of the normal group. The NCV of the high dosage and the drug II group was distinctly faster than that of the model group. The SNCV of the high dosage group was faster than that of the drug II group.6 Expression of NGF Compared with the model group, SJT could obviously increase the expression of NGF in the hippocampus. The best group was the high dosage group.7 Quantitative analysis of myelinated nerve fibers of sural never Compared with the normal group, the fiber number and the fiber density of the model group obviously reduct. That of every treatment group was distinctly more than the model group. The best group were the high dosage group and drug II group.8 Ultrastructure The observation of myelinated nerve ultrastructure showed the model group had greater abnormal ultrastrcture than the normal group and every treatment group in sciatic never including paranodal demyelination, myelin wrinkling, axon atrophg. The high dosage and drugII group could markedly protect ultrastructure of myelinated nerve.Conclusion:SJT can significantly improved the function of nerve and protect the ultrastructure of myelinated nerve. It certainly has protective role on the rats with the early diabetic peripheral neuropathy, deserving to be researched and developed more.