| Objective:Migraine, as a disease of higher incidence, can lead to sharply pain, and break out repeatedly. These often bring great agony to patient, and have a bad effect on their daily life and work. Modern medical science hasn' t totally manifested it' s etiology and pathogenesis, and clinical overall curative effect is on the low side, and it is very difficult to solve recurring. Besides, western medicine has badness and side-effects. However ,traditional Chinese medicine(TCM) can tackle with these problems. Previous clinical research of us have indicated TouTongAn Capsule (TTAC) has certain advantages in alleviating the pain, reducing to break out, preventing recurring and has not obvious poisonous side effects. Therefore, we have designed this experiment to further probe into the function mechanism and offer the scientific basis for TTAC's clinic application and exploitation. Methods:Around TTAC's pharmacology , we use unguentum to carry on little mouse's sprain body test, and super thermostatical water bath device to carry on hot board test, and stereomicroscope to observe little mouse's meningo-microcirculation. According to the relevant requirements of pharmacology toxicity of TCM, we have carried on the acute toxicity test of TTAC. Besides, we have applied nitroglycerin to big mouse to establish the models,and observe TTAC's influence to content of NO in the brain and express of 5-HT, CGRP. Results:1.Among sprain body test, TTAC high dose treatment group, TTAC medium dose treatment group, and Aspirin composita treatment group have been respectively compared with the model contrasting group. we find the former' s incubation period is lengthened, and times of spraining the bodys appear less than the latter. Through statistic analyses, the differences are very obvious (P<0.01); The times of spraining the body of low dose treatment group is less than that of model contrasting group(P<0.05); The times of spraining the body and incubation period of high dose treatment group, compared with Aspirin composita treatment group, have significance differences(P<0.05).2.Among hot board test, TTAC high dose treatment group is compared with model contrasting group, and there is an extraordinary significance difference(P<0.01) . ZhenNaoNing treatment group, compared with model contrasting group at 0.5h after using medicine ,has significance difference(P<0.05), and there are extraordinary significance differences at 1h, 1.5h and 2h after using medicine. TTAC low dose treatment group has obvious difference at 1.5h after using medicine, too.3.Among the experiments of meningo-microcirculation to normal little mouse, wefind TTAC has certain quantity-result relation. TTAC high dose treatment group does not has significance difference to compare with XiBiling.4. After moulding of appling nitroglycerin injecting, we find the contents of NO, CGRP,5-HT in the model big mouse brain have changed obviously: contents of NO and CGRP have remarkably increased. However, content of 5-HT has notably decreased. They have obvious differences(P<0.01), compared with blank contrast group. After using TTAC, positive cell's figure and percentage of high dose treatment group have obviously increased, and there is a significance difference(P<0.01) through comparing with model contrasting group,and there is not obvious difference through comparing respectively with positive contrast group and black contrast group. Positive cell's figure and percentage of 5-HT in medium dose treatment group, compared with model contrast group, have significance difference(P<0.05), and the difference is not obvious through comparing respectively with positive contrast group and blank contrast group. However, positive cell's figure of CGRP in high dose treatment group have obviously decreased, and there is a significance difference(P<0.05) through comparing with model contrast group. The difference of positive cell's figure and percentage between TTAC medium dose group and TTAC low dose group isn' t very obvious(P>0.05), but there is a very remarkable difference(P<0.01) thr... |
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