Rna Interference Induced By Coriaria Lactone Mouse Brain Star-shaped Glial Cells In Multiple Drug Resistance Protein Pgp Expression Inhibition Studies

The mechanism that epilepsy is resistant to drug treatment in about one third of cases is mainly because of the over-expression of multi-drug resistance protein (MDR), which locates on the surface of endotheliocyte of the blood brain barrier. P-Glycoprotein (PGP) is an important member of the MDR family. PGP can pump the anti-epilepsy drug outside of the cell with the energy supply of ATP. RNA interfering is a recently discovered phenomenon that short interfering RNA induces post-transcriptional suppression of gene expression. Our research aimed at downregulating the expression of PGP by way of RNAi to find way to overcome refractory epilepsy. Three couple of siRNA oligonucleotides were designed and cloned into pSIREN vector. We transfected the vectors into astrocytes which were induced to overexpress P-Glycoprotein by Coriaria Lactone. RT-PCR revealed that all the three RNAi-ready pSIREN vectors, especially the No. 1, were able to reduce the mount of rPGP mRNA. The result suggests that RNAi could suppress the expression of PGP. This may be a useful and promising strategy to solve the multi-drug resistance problem in epilepsy patients.