Study Of Selaginella Chemical Composition

This dissertation consists of two chapters:Chapter 1: This chapter includes two sections. The first section is about experiment. The extraction and isolation procedures of chemical constituents in Selaginella tamariscina (Beauv.)Spring was illuminated. An aqueous acetone extract of Selaginella tamariscina(Beauv.)Spring collected in Xixia county, Henan province was suspended in water, extracted by ether, ethyl acetate and n-butanol (saturated with water) respectively. The extract obtained were subjected to a combination of column chromatographies such as silica gel, Diaion HP-20, Toyopearl HW-40, MCI Gel CHP-20, Sephadex LH-20 etc. PTLC was also used to purified the compounds obtained. With such methods, 25 compounds were yielded.The second section describes the elucidation of compounds obtained fromSelaginella tamariscina (Beauv.)Spring. On the basis of spectroscopic analysis andchemical evidences, 20 compounds' structures were determined as β-sitosterol (JB-Y-1),hexadecalic acid (JB-Y-5) , hendecylic acid (JB-Y-6) , amentoflavone (JB-Y-7) ,4-O-[2-hydroxyl-3- (4-hydroxyphenyl) -6-hydroxymethyphenyl]-phenylpropiolic acid(tamariscinic acid A, JB-Y-8), sygringaresinol(JB-Y-9), 2,3-dihydro-5,7,4',5",7",3'",4'"-septahydroxyl-8, 8"-biflavonoids (JB-E-2), robustaflavone (JB-E-4), (7S,8R) -dihydro-7- (3,5-dimethoxyl-4-hydroxyphenyl) -8-hydroxymethyl-3'- methoxyl -1'-acetyl-benzofuran(JB-E-5), neocryptomerin (JB-E-9), genkwanin (JB-E-14), 1 -O-β-D-2-[2-hydroxyl-3-methoxyl-5-( 1 -ethanol )phenyl]-3-( 4-hydroxyl-3,5-dimethoxyphenyl)-propanol-glucopyranoside(tamariscinoside A, JB-A-1) , syringic acid (JB-A-2) , Apigenin-6,8-di-C-β-glucopyranoside(JB-A-3) , caffeoylinositol (JB-A-4) , arbutin (JB-A-5) , 7,8-dihydro-7-(4-hydroxyl-3,5-dimethoxyphenyl)-8-hydroxymethyl -[l'-7'-hydroxyethyl] -5'-methoxyl] benzofuran-4-β-glucopyranoside (tamariscinoside B,JB-A-11), guanosine (JB-A-18) ,1- (4-hydroxyl-3-methoxyphenyl) glycerol (JB-A-19), adenosine (JB-A-20) .Among the 20 compounds, 4-O-[2-hydroxyl-3- (4-hydroxyphenyl) -6-hydroxymethyphenyl] -phenylpropiolic acid (tamariscinic acid A, JB-Y-8), (7S,8R) -dihydro-7- (4-hydroxy-3,5-dimethoxylphenyl)-8-hydroxymethyl-3'- methoxyl -1'-acetyl-benzofuran(JB-E-5),1-O-β-D-2-[2-hydroxyl-3-methoxyl-5- (1-ethanol) phenyl]-3- (4-hydroxyl-3,5-dimethoxyphenyl) -propanol-glucopyranoside (tamariscinoside A, JB-A-1), 7,8-dihydro-7-(4-hydroxyl-3.5-dimethoxyphenyl)- 8-hydroxymethyl-[1'-7'-hydroxyethyl] -5'-methoxyl] benzofuran-4-β-glucopyranoside (tamariscinoside B, JB-A-11) were identified as novel compounds in natural products. The other compounds are all obtained from the plants for the first time except amentoflavone and robustaflvaone.Chapter 2: Determination the quantity of Adenosine in Selaginella tamariscina by RP-HPLC. To establish a RP-HPLC method for the determination of adenosine in Selaginella tamariscina, four samples collected from Xinjiang, Hebei, Anhui and Henan were detected by RP-HPLC. The chromatographic conditions includes SHIM-PACK CLC-ODS column and the mobile phase consisting of a mixture of water-methanol (100:2) for adenosine. The detection wavelength was at 260nm, and measured at room temperature. It has concluded that This method was sensitive, simple and accurate with a good linearity in the concentration range of l~8ug for adenosine. The correlation coefficient was 0.9993. The average recovery of adenosine was 96.70% with 0.93% of RSD.