Post-herpetic Pain Before And After Treatment Proteomic Biomarkers Characteristic Study

Proteomics is a new research area in postgenome era. Its main task is to indentify all proteins in cells,tissues and organisms; its main application aera is medicine matket and mechanism research of diseases. Now a days, the mechanisms of postherpetic neuralgia (PHN) is not known and the developping proteomics gives us a new tool.This article is a part of the research of"Fu Yuan Zhen Tong Soup", which belonged to a great subject of the National Ministry of Education----"the research in the relationship between the function and the drug action foundation of the Chinese medicine"(subject No.00176).1 ObjectiveThis research was designed to study the feasibility of explanation for mechanism of PHN by examing the differences of CSF protein expression of PHN patients after treatment and prepared for further research.2 Contents and methods2.1 Total protein level assay with Ettan 2-D Quant kit.2.2 The CyDyeTM DIGE fluorescent labelling for samples of analysis mode gel. Make use of two dimensional gel electrophoresis of CyDyeTM DIGE fluorescent labelling to determine the differences of protein expression between undergoing PHN patients and cured ones, and find out the differently expressed proteins by fluorescent scanning and distinction analysis.2.3 Mass chromatographic analysis for preparation mode gel. Determine protein species by the application of MALDI-TOF MS.3 ResultsAfter comparisons between photofluorograms by DeCyder analysis softwares, we got 2,500 pretein spots, among which 145 spots are effectively changed. 103 of the them are decreased and 42 are increased in concentration. We take one of the proteins named Protein A for example. After digestion,MALDI-TOF-MS analysis and searching database, we confirmed the name of Protein A as Complement factor B. Besides this protein, 32 proteins were identified.4 ConclusionsDifferently expressed proteins can be found out by using proteomics methods. After mass analysis and searching the database, we could find out the exact protein. The results of this paper demonstrate the feasibility of proteomics technogy in PHN studies and this paper also prepared for further research in DSPs of PHN. 5 New ideas5.1 The application of the fluorescent labelling in proteomics in the mechanism research in PHN.5.2 The application of proteomics in the identification of the information substances in Chinese medicine.