| The Chinese herb Viola yedoensis Makino is used common treat furunculosis on the clinic, it have longtime officinal history and have complex variety. People unable to decide which is quality goods for a long time. Along with quality standardization of Chinese herbs continue to extend, it is necessary to study the chemical composition and establish quality standard. Therefore researching and exploring the true meaning, discussing the variety, studying the chemical composition and quantitative analysis of the effective composition,as a whole providing the gist for based quality standard.This article include neoteric literature summarize, literature review and variety dissertation, study of the chemical composition and quantitative analysisof the effective composition. Chapter 1 In this chapter, we made an overview of the Viola yedoensis Makino. Including: 1. Study of Viola yedoensis Makino from the literature review of herbs. 2. Chemical composition general reserch contains that Viola yedoensisMakino and Viola prionantha Bge of the Viola, as well as substitute Gueldenstaedtia Multiflora Bge and Corydalis bungeana Turcz for Viola yedoensis Makinoin different areas. 3. The current pharmacology study of Viola yedoensis Makino. 4. Traditional and current clinical application study on Viola yedoensis Makino. 5. Study of the quantitative analysis of the effective composition.Chapter 2 In this chapter we study Viola yedoensis Makino on the literature review of herbs and variety dissertation.1. Base the literature review age of the herbs on the clue, combine the variousbooks and related literature, the"Jin"originated from the plant of Viola. Studying its rule of the development and change, we found that the plant of Viola's application ascend to the Zhou period, however as normal official plant,it has been appeared in the《fifty-two bing fang》of the Han period. Thereout we educed as the same category plants Viola yedoensis Makino's history that from appearance to variety complex.2. Through the archaic and nowadays literature recordation, we brief discussed other category which is used on the clinic in common, made a good foundation for the next step to study on its complex variety.Chapter 3 Study on Viola yedoensis Makino's chemical composition. Various chromatographic, spectral and chemical methods are used to isolate the effective composition. It shows that the Viola yedoensis Makino is composed of the organic acid and flavonoid glycoside. As a result,14 compounds were isolated from the Viola yedoensis Makino. 12 of them were evaluated. They are chikusetsusaponin-Ⅵa(1),vicenin-2(2),apigenin-6-C-β-D-glucopyranosyl-8-C-α-L-arabinopyranoside(3),apigenin-6,8-di-C-α-L-arabinopyranosyl(4),Hexadecanoic acid(6),Hendecanoic acid(7),Dodecanoic acid(8),Decanoic acid(11),Ceratinic acid(12),β-sitosterol(9),D-Glucose(13) and Sucrose(14).Thereinto,compounds 1,7,8,9,11,12,13,14 are isolated from this plant for the first time.Chapter 4 Study on the Viola yedoensis Makino's quantitative analysis of the effective composition. The contents of index compounds including vicenin-2 and apigenin-6,8-di-C-α-L-arabinopyranosyl are determined by HPLC. As a result, the calibration curves(y=655.18x+4.2992)are linear at ranges of 0.040~1.550μg(for vicenin-2, R2=0.9991). And the content was 0.41mg/g, the average recoveries were 98.21%(RSD=1.7%, n=6).The calibration curves (y=904.56x+10.786)are linear at ranges of 0.055~2.150μg(for apigenin-6,8-di-C-α-L-arabinopyranosyl R2=0.9997). And the content was 0.71mg/g,the average recoveries were 99.09%(RSD=1.87%, n=6).Chapter 5 Analysis and Summary... |
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