Shulie An Raw Materials Of Wool Hangzhou Sub-tip Active Ingredient

With the global trends in aging,the incidence of prostate diseases is stably increasing.How to prevent and treat these diseases has been an important topic in the medical field for old men. To fred safer and more effective agents from natural products attracts more and more attention.In this thesis,we tested parts of traditional Chinese agents on sale used for the treatment of benign prostate hyperplasia(BPH)by evaluating their inhibition effect on secreted prostate specific antigen(PSA)in LNCaP cells with ELISA method,and found ShuLieAn showed strong activity.The raw material plant of Shuliean is Campylotropis hirtella(Franch.)Schindl.We found that the n-butanol extract of Campylotropis hirtella was one of the active fraction of the whole plant,and further carried out active constitutes study on the fraction by bioassay-guided isolation.18 compounds from the n-butanol extract of Campylotropis hirtella were isolated through various chromatographic techniques.17 of their structures were elucidated on the basis of physico-chemical property and spectroscopic analysis.The 17 compounds were Citroside A (1),2,7,4'-trihydroxyflavanone-5-O-β-D-glucopyranoside(2~*),orientin(3),isoorientin(4), viexin(5),isovitexin(6),vitexin-2"-xyloside(7),rutin(8),quereetin-3-O-β-D-glucopyranoside (9),3,5-dimethoxy-4-hydroxyphenol-1-O-β-D-apiofuranosyl-(1â†'6)-O-β-D-glucopyranoside (10),2,6-dimethoxy-4-hydroxyphenol-1-O-β-D-glucoside(11),3,4,5-trimethoxyphenyl-1-O-β-D-glucopyranoside(12),benzyl-O-β-D-glueopyranoside(13),syringaldehyde(14), (-)-secoisolariciresinol-O-β-D-glucopyranoside(15),(E)-resveratrol-3,5-O-β-D-diglucopyrano -side(16),daucosterol(17),respectively.Compounds 2 is a new compound.Compounds 1,3,5, 7,10,11,12,14,16 were isolated for the first time from the Campylotropis genus.