P53, EGFR And Expression Of PCNA In Thymoma And Its Clinical Significance

Objective:To study the expression of P53, EGFR and PCNA in thymoma and to explorethe correlation between the expression of the factors and the thymoma status,including pathological types, clinical stage and differentiation, MG combine andprognosis .Methods:Surgically resected and pathologically proved cases of thynoma werecollected. These patients have been followed up for 3 years by questionnaire andphone calls, all of clinical and follow-up information were included in the study,and all the patients received neither radiocherapy nor chemocherapy before thesurgery. Information related to patient's histological type, pathological stage andsurive time were recorded. The expression of P53, EGFR and PCNA weredetected by immunohistochemistry SP method. All the results were statisticallyanalyzed with SPSS 12.0 software package.Results:The expression of P53 was significantly different between thymoma and thetissue of normal thymus (P<0.05). Different ways of expression was revealed indifferent stages of thymomas. The expression of P53 was significantly different indifferent Masaoka stage and WHO histological classification (P<0.05). Theexpression of P53 wasn't different in Bernatz histological classification andwith/without MG(P>0.05). The expression of EGFR and PCNA was significantlydifferent in different Masaoka stage and WHO histological classification (P<0.05).The expression of EGFR and PCNA wasn't different in Bernaze histologicalclassification and with/without MG(P>0.05). There also has a lower survival ratein tissues with expression of P53, EGFR and PCNA than that without expressionof P53, EGFR and PCNA(P<0.05).Conclusion1. Along with upstage of thymoma's Masaoka and WHO classification , theexpression of P53, EGFR and PCNA was increased. 2. Expression of P53, PCNA and EGFR protein wasn't correlated with WHOand Bernatz histological classification and with/without MG. It was correlated withMasaoka stage and TNM. It suggested that they can better reflect malignent degreeand activity of growth of malignent thymoma.3. Expression of P53, PCNA and EGFR correlate with histological grade andTNM stage suggested that P53, PCNA and EGFR can used as an objective indexfor prognosis in invasive thymoma.