Traditional Chinese Medicine Effective Parts Of The Crane Compound Group And Its Anti-tumor Effect

"Developing the effective fraction group of Chinese Traditional Compound Medicine(CTMC) is an available way to the modernization of Chinese herbal, we should carry on the serial research of Chinese herbal and CTMC at the basis of elucidating the curative action and mechanism." This is raised by relevant experts at "The Third Profound Scientific International Seminar of the Protection and utilization of Biodiversity". The effective fraction extracted from CTMC has been removed many components with adverse reaction, its curative action is increased and adverse reaction decreased; chemical constituents are more clearly, can be easily formulate the quality standard and production.On the bases of these viewpoints, a Chinese Traditional Compound Medicine—Xianhe consisting of Agrimonia pilosa Ledeb. Hedyotis diffusa Willd, Solanum lyratum Thurb and Glycyrrhiza uralensis Fisch, was systematical studied to separate the effective fraction guided by the antitumor or effective fractions—polysaccharide, water-solubility saponin, flavonids, liposolubility saponin .To establishment of a Chinese medicine compound effective crane parts extraction and purification process by extract yield and effective site for the content of indicators; the crude extracted saponin of alcohol extract of fat-soluble saponin by use of ether precipitated and establishment the purification of saponin effect and purification conditions of the different models of the macroporous resin .By use of the method of base extract and acid precipitated to extract total flavonoids and establishment the artwork condition of the PA column chromatography separation and purification of total flavonoids Technology; the extraction of compound prescription total polysaccharide by use of water extract after alcohol extract, a typeⅡZTC1 +1 Natural clarification on the application of the purification of total polysaccharide and compared to traditional water-extraction; The effective parts of the compound of different strains of IC50 are determinated by MTT in vitro; To establish a mouse model of liver cancer H22 could be verified from the compound in the separation of the effective parts and combinations of anti-tumor effect;At last, With a weight of the compound effective method of allocation of parts of the group to optimize the dose and find the best.This article identify the Chinese crane effective parts of the compound, and abstraction and separation, purification and characterization identification, content determination, pharmacodynamics and research of other aspects . The results show that the total polysaccharide, water-soluble saponin, total flavonoids, saponins fat-soluble extract separation, purification programme feasible, in vitro and in vivo anti-tumor effect results showed that the crane and its compound effective parts of pharmacodynamics group with a clear and stability.